A) Write out both strands of the recognition sequences for BamHI and Sau3A, show what they would look like after they are cut, and what the DNA would look like after you ligate a BamHI site to a Sau3A site. Make sure that it is easy to follow the individual pieces of DNA. You may want to use two different colors to keep them separate. B) What fraction of BamHI sites can be cut by Sau3A? What fraction of Sau3A site can be cut by BamHI? C) If two BamHI sites are ligated together, the resulting site can be cleaved again by BamHI. The same is true of two Sau3A ends. Suppose you ligate a fragment with a Sau3A end into a plasmid that has been cut by BamHI, can the hybrid site be cut with BamHI? Can it be cut with Sau3A? D) Restriction enzyme recognition sites occur randomly throughout the genome. Calculate the probability of a Sau3A site (each nucleotide has 4 possibilities and in statistics the probability of multiple events is the probability of one multiplied by the probability of the other). Is that greater or less than the probability of a BamHI site? What is the probability of a BamHI site?

A) Write out both strands of the recognition sequences for BamHI and Sau3A, show what they would look like after they are cut, and what the DNA would look like after you ligate a BamHI site to a Sau3A site. Make sure that it is easy to follow the individual pieces of DNA. You may want to use two different colors to keep them separate. B) What fraction of BamHI sites can be cut by Sau3A? What fraction of Sau3A site can be cut by BamHI? C) If two BamHI sites are ligated together, the resulting site can be cleaved again by BamHI. The same is true of two Sau3A ends. Suppose you ligate a fragment with a Sau3A end into a plasmid that has been cut by BamHI, can the hybrid site be cut with BamHI? Can it be cut with Sau3A? D) Restriction enzyme recognition sites occur randomly throughout the genome. Calculate the probability of a Sau3A site (each nucleotide has 4 possibilities and in statistics the probability of multiple events is the probability of one multiplied by the probability of the other). Is that greater or less than the probability of a BamHI site? What is the probability of a BamHI site?

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter14: Dna Structure And Function

Section: Chapter Questions

Problem 34CTQ: Quinolone antibiotics treat bacterial infections by blocking the activity of topoisomerase. Why does...

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