A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate. Plate 1 – 242 colonies Plate 2 – 237 colonies Plate 3 – 249 colonies Calculate the CFU/mL for this juice. Show formula used and include units
Q: A nurse colleague asks for your help in drawing blood cultures from a patient with suspected…
A: Bacteremia is when bacteria circulate through the bloodstream. It can be caused by everyday habits…
Q: Do you think standard plate counts are very accurate? Why or why not? When doing serial dilutions,…
A: You have asked multiple questions. I will answer the first 3 subpart question, as allowed by…
Q: samples and trying to determine characteristics they contain. You determine sample M requires an…
A: On the basis of Habitat in which the microorganism live, these can be classified into many types.…
Q: a food sample with an unknown number of contaminating bacteria was serial diluted to 10-6. the 10-6…
A: Serial dilution is a process of diluting a particular sample in a solution. The purpose of doing a…
Q: You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria…
A: The bacteria can grow only in the favourable conditions that is when there is optimum amount of pH,…
Q: Per the USDA, whole, unpasteurized fresh eggs can contain no more than 50,000 CFU/mL bacteria in a…
A: The serial dilution is a process that is routinely used in microbiology laboratories for the…
Q: The number of bacteria in the samples need to be quantified. The laboratory uses a specific protocol…
A: Introduction :- Bacteria are single-celled organisms with a small size. Bacteria can be found…
Q: Students in a microbiology lab are provided with a culture of E. coli, and don't know how long it's…
A: Bacterial growth is increase in the quantity of cellular constituents and number .It depends upon…
Q: Calculate the coliform concentration in a milk sample based on the following colony counts: 10 2 :…
A: Introduction Coliform bacteria are defined as rod-shaped Gram-negative non-spore forming and motile…
Q: 2. A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice.…
A:
Q: Pretend the results below were obtained for water from your well. The 10 mL tubes are Full Strength,…
A: Most Probable Number or MPN method is a way to estimate the number of microorganisms in the water…
Q: You begin with an overnight culture of approximately 108 organisms/ml and need a concentration of…
A: In an Overnight culture of the bacteria, their growth factor depends upon the type of bacteria we…
Q: A serial dilution of a sample has an unknown concentration of microorganisms. A countable plate has…
A:
Q: A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice.…
A: Colony forming units (CFU) indicates the count of individual colonies of a microorganism that is…
Q: Vernon wanted to determine the colony forming units of bacteria per gram of soil in their school…
A: CFU (colony-forming unit) or cfu (colony-forming unit) is a metric for the number of viable…
Q: You have a cell count of 6.7 x 10° cells / ml in a total volume of 4.5ml. You have resuspended your…
A: The concentration of bacteria depends on many factors like inoculum concentration, incubation…
Q: An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100…
A: Serial dilution is used to find the concentration of bacteria in the sample in case of greater…
Q: Kousei works at a company that produces sweetened condensed milk. If one or more samples exceed 10…
A: Cell count: The cell count data is generally utilized to predict the number of living microbes…
Q: Answer each of the questions below using the plate images provided. Nutrient Agar 1 EMB Blood Agar…
A: EMB stands for Eosin Methylene Blue. EMB is a stain that is selective for gram-negative bacteria.…
Q: A 50 mL sample of soup was found to be contaminated with E. coli. A dilution of the soup was…
A: Procedure to find CFU- 1. Dilute the sample 2. Plate the dilutions with correct growth medium 3.…
Q: The number of bacteria in saliva samples was determined by collecting the saliva, making serial…
A: Microorganisms are minute organisms that cannot be seen by naked eyes. They can be visualized only…
Q: You have 0.05 ml of kombucha that contains microbes at a concentration of 8.2 x 10^6 CFU/ml. You…
A: Creating proper dilutions and working out the colony-forming units or CFU/ ml is one integral part…
Q: Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions…
A: Note- we are supposed to answer three subpart of a question according to our guidelines. Please…
Q: Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment…
A: The equipment used in microbiology labs comprise a wide range of instruments that are necessary for…
Q: A microorganism was streaked onto 4 nutrient agar plates and the plates were incubated at different…
A: Nutrient agar A type of general media that supports growth of wide variety of microorganisms.
Q: neration time of the species is 1 hour, and the microbes are in the exponential (log) hase of…
A: Microorganism that experience exponential growth its number increases according to this equation…
Q: You attempted to determine the number of bacteria in a culture using the Turbidimetry method with a…
A: Bacterial cell counts in a broth or culture can be determined by culturing multiple plates and…
Q: Determine the number of bacterial cells per ml. in the original culture. 1ml 1ml 1ml 1ml 99ml 99ml…
A: Serial dilutions are used to minimize bacterial concentrations to a level necessary for a certain…
Q: True or False: An isolated bacterial colony supplies cells.to grow "pure culture" of the bacteria.…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: Use the measurement tool to measure the zone of inhibition for each antibiotic sample in millimeters…
A: Sample A: 32.48mm , 32.5mm, 32.5mm , Average : 32.5 mm Sample B:. 24.6mm , 24.7mm , 24.7mm…
Q: Determine the number of bacteria per ml. of water specimen. 1ml 1ml 1ml water specimen H,0 blanks…
A:
Q: You were tasked to determine the total microbial load of a bread sample. The sample was homogenized,…
A: Question: Draw a schematic diagram illustrating the proposed experimental setup described above?
Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…
A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…
Q: The following data were obtained from a test in which growth was first assessed in the presence of…
A: The answer for the present MCQ has been explained with definition and in the following step.
Q: Using saline tubes containing 9 ml of saline (for dilutions), draw a diagram that would yield…
A: All the bacteria in a colony are identical as they arise from a single bacterium. Thus, a colony is…
Q: A student is performing a bacterial growth curve experiment. At t= 0 hrs, he inoculated 50 mL of the…
A:
Q: Which laboratory procedure is represented in the diagram below? Paper towel 1) reducing the size of…
A: Often microscopic examination of a specimen involves many procedures to be followed, from slide…
Q: about how cultures are handled in a clinical laboratory
A: Microbial cultures are wont to confirm the sort of organism, its abundance within the sample being…
Q: A urine sample is diluted 1:1,000. If 0.1 ml were put into a Petri dish with melted agar, how many…
A:
Q: You were tasked to determine the total microbial load of a bread sample. The sample was homogenized,…
A: Serial dilution is a step-wise dilution of microorganisms in the solution. The dilution factor…
Q: Determine the number of bacteria per ml. of water specimen. 1ml 1ml 1ml water specimen H,0 blanks…
A: The aim of the serial dilution approach is to estimate the concentration of an unknown sample…
Q: The plates below were each created with 0.5 mL of culture from a 10-fold dilution series. Each plate…
A: Serial dilutions are utilised for the calculation of the concentration of microorganisms. Since it…
Q: A SAB agar plate is exposed to air for 30 minutes and then incubated. The 10 colonies which grew on…
A: Sabouraud Dextrose Agar (SDA) is used for the isolation, cultivation and maintenance of…
Q: Observations and Results Average Count per ml of Sample (CFU/ml) Bacterial Dilution Factor ml of…
A: Introduction The Stepwise Dilution Of A Substance In Solution Is Referred To As Serial Dilution.…
Q: A 24-hour broth culture of E coll is estimated to have 75x10 cells.If you were to do a plate count…
A: bacteria such as E.coli is known to divide every twenty minutes. This means the number of cells…
Q: You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions…
A: Hi, since there are multiple questions posted we will provide you with answers for the first…
Q: s shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number…
A: CFU count is the technique of enumeration of microbial cells in a sample and it is one of the most…
Q: 1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to…
A: A bacteria culture is described as a type of test that is used to confirm whether you have a…
Q: Below is a diagram of the serial dilution of a culture with 1 x 10° cells. Fill out the missing…
A: Colony-forming unit / mL= Number of colonies * Dilution factor * 1/volume plated in mL CFU/mL = 1 *…
A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate.
Plate 1 – 242 colonies
Plate 2 – 237 colonies
Plate 3 – 249 colonies
Calculate the CFU/mL for this juice. Show formula used and include units
Step by step
Solved in 2 steps with 3 images
- A laboratory technician performed a series of 10 fold serial dilutions shown below. he plated 0.1 mL from each dilution tube onto NA and got the follow numbers per plate dilution 1:10 1:10 1:100 1:1000 1:10000 1:100000 colony count >1000 >1000 808 303 38 3 calculate the concentration of bacteria in the undiluted stock (in CFU/mL)Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uLA researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate. Plate 1 – 242 colonies Plate 2 – 237 colonies Plate 3 – 249 colonies a. Calculate the CFU/mL for this juice. Show formula used and include units. b. If acceptable levels of E. coli in juice were 0.02 CFU/ml juice, would this juice be acceptable for human consumption? Why or why not?
- The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution -5 Plate 3 10 dilution Plate 4 10 dilution Plate 5 107 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* TMTC* TMTC* 867 154 18
- The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution -3 Plate 1 10 dilution Plate 2 10-4 dilution Plate 3 10-5 dilution Plate 4 10 dilution Plate 5 10 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 1,957 65 21 1 0 m1mL Stock #1 1mL 9mL #2 9mL #3 1mL A 9mL #4 0.1mL 1mL O O B 9mL #5 1mL 1mL 9mL #6 O Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units. 0.1mL 1mk D OTen grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?
- You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the undiluted milk, you do serial dilutions, and plate 0.5 ml of 4th dilution on agar. If you counted 78 CFUs from the 4th plate, how many bacteria/ml do you expect? (show solution) Do you think standard plate counts are very accurate? Why or why not?You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution schemeDetermine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6