You were tasked to determine the total microbial load of a bread sample. The sample was homogenized, and 25 g of sample was weighed. This was immediately placed in a flask containing 225 mL diluent and gently shaken to mix. From this, 10 mL was taken and transferred to a 90 mL diluent. A 10-fold* serial dilution was performed and samples from 10-4, 10-5 and 10-6 was pour-plated in triplicates. *the final total volume is 10 mL a.Calculate the total volume (mL) of culture media needed for you to be able to perform the said experiment. Show your solution.
You were tasked to determine the total microbial load of a bread sample. The sample was homogenized, and 25 g of sample was weighed. This was immediately placed in a flask containing 225 mL diluent and gently shaken to mix. From this, 10 mL was taken and transferred to a 90 mL diluent. A 10-fold* serial dilution was performed and samples from 10-4, 10-5 and 10-6 was pour-plated in triplicates. *the final total volume is 10 mL a.Calculate the total volume (mL) of culture media needed for you to be able to perform the said experiment. Show your solution.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You were tasked to determine the total microbial load of a bread sample. The sample was homogenized, and 25 g of sample was weighed. This was immediately placed in a flask containing 225 mL diluent and gently shaken to mix. From this, 10 mL was taken and transferred to a 90 mL diluent. A 10-fold* serial dilution was performed and samples from 10-4, 10-5 and 10-6 was pour-plated in triplicates.
*the final total volume is 10 mL
a.Calculate the total volume (mL) of culture media needed for you to be able to perform the said experiment. Show your solution.
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