Per the USDA, whole, unpasteurized fresh eggs can contain no more than 50,000 CFU/mL bacteria in a standard plate count. You are curious if the fresh eggs that you buy from your neighbor are considered safe to consume so you use your eScience Microbiology kit to test these eggs using direct plate count after serial dilution. After you complete the experiment, you obtain 74 countable colonies from the 10-2 dilution plate. The inoculum volume you plated was 0.1 mL.   How many bacteria are present in 1 mL of the egg you sampled?   Are these eggs considered safe to consume per USDA standards?

Q: A food sample with an unknown number of contaminating bacteria was serially diluted to 10-6. A…

A: Serial dilution of a food sample containing unknown quantity of contaminating bacteria was performed…

Q: student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step…

A: 5. The loop was flamed

Q: If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original…

A: CFU stands for colony-forming unit that gives rise to a particular colony. It is generally assumed…

Q: One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies…

A: A colony-forming unit, or CFU, is a measurement of the concentration of microorganisms in a test…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: A culture of E. coli has a concentration of 5 x 108 cells/mL. How many times do you have to dilute…

A: Given: 1. Number of colonies: 2502. No. of bacteria in culture: 5 X 108 3. Volume plated: 0.1 ml 4.…

Q: a food sample with an unknown number of contaminating bacteria was serial diluted to 10-6. the 10-6…

A: Serial dilution is a process of diluting a particular sample in a solution. The purpose of doing a…

Q: Two different bacterial samples, A and B, were inoculated onto SIM media. What can you conclude…

A: SIM (Sulfide, Indole and Motility) test is done to differentiate the Gram negative bacilli based on…

Q: how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.

A: Introduction Coliform is the gram-negative, non-spore-forming and motile or non-motile bacterial…

Q: You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of…

A: S.Aureus is the most common cause of abscesses, furuncles, and cellulitis in the skin and soft…

Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: 1 ml from main culture 1ml 99999 5 ml LB 1 ml 5 ml LB 5ml LB 1ml 5 ml LB 1 ml 5 ml LB You took 250…

A: To calculate the number of cfu/ml bacteria in the sample, we need to use the dilution factor and the…

Q: You found a bright blue growth on a tomato you had left on your kitchen counter. You’ve never seen a…

A: Introduction  A hypothesis is a proposed explanation for a phenomenon or observation that can be…

Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…

A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…

Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: Step #1 Culture and perform a Gram-stain on the Super Bug You culture the Super Bug by swabbing the…

A: Bacteria are simple unicellular prokaryotic microorganisms that lack membrane bound organelles and…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

A: Fibronectin is a glycoprotein found in the extracellular matrix of cells and is also obtained from…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells.…

A: The passage number of a cell culture is a record of the number of times the culture has been…

Q: You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you…

A: Introduction There are different culturing techniques such as agar plates, broth culture, stab…

Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

A: A colony-forming unit (cfu) is a unit used in microbiology to estimate the number of viable bacteria…

Q: Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three…

A: E.coli cells take around 20 mins to replicate themselves. The replication of bacteria is an…

Q: What is science

A: Science is an  attempt to make the Chaotic diversity of our sense experience corresponds to logical…

Q: You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this…

A: Bacteria are prokaryotic organism and divides by binary fission that is responsible for doubling the…

Q: Below is a set of data for a serial dilution/plate count experiment.    Based on this data what is…

A: There are numerous methods/techniques for counting the number of bacteria in a particular sample. A…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…

A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…

Q: Based on these results, which of the following statements is true regarding the effectiveness of…

A: A disinfectant is a chemical substance or compound that is used to inactivate or destroy the…

Q: A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture…

A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting…

A: Dilution : We know that the solute of the concentration will be decreasing in solution . There is a…

Q: Joseph, being a know-it-all, told Mary that her method would not include all microbes. To…

A: Joseph and Mary had a disagreement about the effectiveness of Mary's method for counting microbes in…

Q: You are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase…

A: Bacterial culture: It is a technique that enables the controlled multiplication of bacterial cells…

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: This is how a hanging-drop slide is prepared. Figure from Macedo, Wikimedia Commons, 2016. 2 3 4…

A: Observing and studying living microorganisms, such as bacteria or protozoa under a microscope can be…

Q: I completed the chart, and I'm supposed to figure out the unknown bacteria, there are 12 unknown…

A: AnswerBased on the information provided in your chart, let's compare the characteristics of your…

Q: One hundred microliters (0.100 ml) of a 10-5 dilution of a bacterial suspension plated on an agar…

A: Colonies of bacteria live on solid media. A colony is characterized as a detectable microorganism…

Q: According to these images and your own knowledge, can you make a biological drawing of bacteria in…

A: Yoghurt is a nutrient which is rich in lactobacillus (lactic acid bacteria) . Convert milk into…

Q: A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From…

A: Serial dilution is a process by which a substance such as a bacterial culture is diluted stepwise in…

Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

A: Minimal medium (unsupplemented) A minimal medium is a growth medium used for growing microbes and…

Q: This test determines the ability of bacteria to use citrate as source of carbon and inorganic…

A: The citrate test is utilized to check whether a bacterium species can use citrate as a source of…