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Tell me three things about how cultures are handled in a clinical laboratory.
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- Tell me three things that you know about safety in a lab in general and what you would like to learn about safety in the microbiology lab.Why are there strict rules and policies when it comes to working in a laboratory? What would happen if you did not follow the guidelines? What could happen to the samples? The patient? What do you think would be the right course of action?What will pose an ethical issue in these procedures are performed - sterilization?
- What will pose an ethical issue in these procedures are performed - mutilation, sterilization?You have learned many different techniques so far this semester that allow us to identify pathogenic microbes in the clinical setting. Why do you think rapid methods are so helpful? Why are traditional tests such as metabolic tests not always used to ID clinically important microbes?What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
- Which one of the following microorganisms would you use as a quality control check to ensure that your sterile technique method is properly working? S. aureus, a gram-positive bacterium E. coli, a gram-negative bacterium B. cereus, an endospore-forming bacterium M. leprae, an acid-fast bacterium T. pallidum, a flagellated, disease causing bacteriumIdentification of an organism does not require a pure culture because Bergey’s Manual will sort out the contaminant. Identification of an organism does not require a pure culture because Bergey’s Manual will sort out the contaminant. True FalseA student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celcius
- Why are specimens for bilirubin testing protected from light? Discuss handling procedures for bilirubin samples.Tell me three things that you know about what you know about "clinical microbiology?" What is covered in this branch of microbiology and how will this relate to a nurse working in a doctor's office or hospital?Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample, added urea, and then let it incubate for one week. She then performed serial dilution a shown below: 10 g soil sample To: 1ml 90 ml Dilution Bottle 1 ml 9 ml Tube 1 0.1 ml 0.45 ml 111 9.9 ml Tube 3 9 ml Tube 2 9.55 ml Tube 4 Calculate the dilution of the bottle and each tube. Be sure to show complete solutions and box your final answer.