The plates below were each created with 0.5 mL of culture from a 10-fold dilution series. Each plate is marked with the dilution as well as the number of colonies that were counted. Pick a plate that has a countable number and calculate the CFU/mL for the original culture. Include units in your answer and show your work.
The plates below were each created with 0.5 mL of culture from a 10-fold dilution series. Each plate is marked with the dilution as well as the number of colonies that were counted. Pick a plate that has a countable number and calculate the CFU/mL for the original culture. Include units in your answer and show your work.
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![**Determining CFU/mL from a 10-Fold Dilution Series**
The plates below were each created with 0.5 mL of culture from a 10-fold dilution series. Each plate is marked with the dilution as well as the number of colonies that were counted. Pick a plate that has a countable number and calculate the CFU/mL for the original culture. Include units in your answer and show your work.
**Steps:**
1. **Select a Countable Plate:**
- Choose a plate with colonies that are within the countable range (typically 30-300 colonies).
2. **Calculate CFU/mL using the Formula:**
\[
\text{CFU/mL} = \frac{\text{Number of Colonies}}{\text{Volume Plated (mL)} \times \text{Dilution Factor}}
\]
3. **Units and Work:**
- Clearly state all units in your calculations.
- Show each step in your calculations to ensure accuracy and transparency.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1131871b-8c87-406d-9807-6125c8d6ca38%2Fc81fb691-1f72-4aa2-ad2c-35d66a8e20fb%2Fi6ojfql_processed.jpeg&w=3840&q=75)
Transcribed Image Text:**Determining CFU/mL from a 10-Fold Dilution Series**
The plates below were each created with 0.5 mL of culture from a 10-fold dilution series. Each plate is marked with the dilution as well as the number of colonies that were counted. Pick a plate that has a countable number and calculate the CFU/mL for the original culture. Include units in your answer and show your work.
**Steps:**
1. **Select a Countable Plate:**
- Choose a plate with colonies that are within the countable range (typically 30-300 colonies).
2. **Calculate CFU/mL using the Formula:**
\[
\text{CFU/mL} = \frac{\text{Number of Colonies}}{\text{Volume Plated (mL)} \times \text{Dilution Factor}}
\]
3. **Units and Work:**
- Clearly state all units in your calculations.
- Show each step in your calculations to ensure accuracy and transparency.
Transcribed Image Text:This image displays a series of petri dishes used to illustrate bacterial colony counts at various dilution levels. Below is a detailed explanation of the image:
1/10 dilution: Uncountable colonies.
1/100 dilution: Uncountable colonies.
1/1000 dilution: Approximately 1.7 x 10^4 colonies.
1/10,000 dilution: Approximately 1.2 x 10^2 colonies.
1/100,000 dilution: 197 colonies.
1/1,000,000 dilution: 10 colonies.
### Petri Dish Descriptions:
- **1/10 Dilution:**
- The top left petri dish shows a large number of bacterial colonies, making it difficult to count individual colonies accurately.
- **1/100 Dilution:**
- Similar to the 1/10 dilution, the number of bacterial colonies is too high for an accurate count.
- **1/1000 Dilution:**
- The number of colonies has reduced, but it is still relatively high, around 1.7 x 10^4 colonies.
- **1/10,000 Dilution:**
- The dilution has significantly reduced the bacterial count, making it approximately 1.2 x 10^2 colonies.
- **1/100,000 Dilution:**
- The petri dish shows 197 colonies, indicating a substantial reduction in the bacterial load.
- **1/1,000,000 Dilution:**
- The final petri dish shows 10 bacterial colonies, demonstrating a clear dilution factor leading to a manageable number of colonies for counting.
This sequence visually illustrates how serial dilutions can effectively reduce bacterial concentrations to quantifiable levels, a common practice in microbiological studies and experiments.
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