A piece of DNA fragment is sequenced. You clone the fragment, isolate the cloned DNA fragment, and set up a series of four dideoxy reactions. You then separate the products of the reaction by gel electrophoresis and obtain the following banding pattern:- **ddATP lane:** - Band 1 (top) - Band 5 (bottom)- **ddTTP lane:** - Band 2- **ddCTP lane:** - Band 3 - Band 6 (bottom)- **ddGTP lane:** - Band 4 - Band 7 (bottom)**Question:** What is the base sequence of the original fragment that you were given?**Options:**1. 5'-TAAGCTGA-3'2. 5'-ATTCGACT-3'3. 5'-TCAGCTTA-3'4. 5'-AGTCGAAT-3'

While deciphering a sequencing gel, we know that it depends on terminating the reaction at 3' end…

A piece of DNA fragment is sequenced. You clone the the fragment, isolate the cloned DNA fragment, and set up a series of four dideoxy reaction. You then separate the products of the reaction by gel electrophoresis and obtain the following banding patter: ddATP ddTTP ddCTP ddGTP What is the base sequence of the original fragment that you were given? 0 5-ТАAGCTGA-3' 0 5-АТTCGACТ-3' 5'-TCAGCTTA-3' 5'-AGTCGAAT-3'

A piece of DNA fragment is sequenced. You clone the the fragment, isolate the cloned DNA fragment, and set up a series of four dideoxy reaction. You then separate the products of the reaction by gel electrophoresis and obtain the following banding patter: ddATP ddTTP ddCTP ddGTP What is the base sequence of the original fragment that you were given? 0 5-ТАAGCTGA-3' 0 5-АТTCGACТ-3' 5'-TCAGCTTA-3' 5'-AGTCGAAT-3'

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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