7. You are studying the folding and stability of a bacterial nuclease. Upon exposing the enzyme to 8 M urea and mercaptoethanol, you find that its activity to hydrolyze DNA drops from 100% to -0.01%. After removal of the mercaptoethanol, you find that the enzyme's activity remains close to -0.01%. Next, you remove the urea and find that the activity is restored to -33%. However, if you perform an equivalent experiment but remove both mercaptoethanol and urea at the same time, the activity of the enzyme is restored to -98%. Provide a biochemical explanation for these findings.
7. You are studying the folding and stability of a bacterial nuclease. Upon exposing the enzyme to 8 M urea and mercaptoethanol, you find that its activity to hydrolyze DNA drops from 100% to -0.01%. After removal of the mercaptoethanol, you find that the enzyme's activity remains close to -0.01%. Next, you remove the urea and find that the activity is restored to -33%. However, if you perform an equivalent experiment but remove both mercaptoethanol and urea at the same time, the activity of the enzyme is restored to -98%. Provide a biochemical explanation for these findings.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:7. You are studying the folding and stability of a bacterial nuclease. Upon exposing the enzyme to 8 M
urea and mercaptoethanol, you find that its activity to hydrolyze DNA drops from 100% to -0.01%.
After removal of the mercaptoethanol, you find that the enzyme's activity remains close to -0.01%.
Next, you remove the urea and find that the activity is restored to ~33%. However, if you perform an
equivalent experiment but remove both mercaptoethanol and urea at the same time, the activity of the
enzyme is restored to ~98%. Provide a biochemical explanation for these findings.
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