7. A peptide has the sequence Cys-His-Glu-Met-lle-Ser-Thra. Write out the single letter sequence of this peptide.b. What is the overall charge of the peptide? Is it acidic? Basic?c. Draw the chemical structure of the peptide at pH 7.0 and show any chargedgroupsd. What is the classification of each amino acid in this peptide regardingpolarity and charge?8. The following proteins represent a wide range of molecular weights andisoelectricpoints. Mr is the molecular weight of a single protein chain.• Protein 1: Mr 68,544; pl 6.11 (monomer)• Protein 2: Mr 29,041; pl 5.32 (dimer)Protein 3: Mr 15,805; pl 5.7 (dimer)• Protein 4: Mr 12,165; pl 4.74a. Which protein is the most acidic? Explain your answer.b. Which protein will migrate the slowest in an SDS-PAGE? Explain youranswer.c. In what order will these proteins elute from a cation exchanger at phH 8?Explain your answer.d. In what order will these proteins salt out from a pH 7 solution by thedropwise addition of saturated ammonium sulfate? Explain your answer.

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Answered: 7. A peptide has the sequence…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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1. Consider the peptides P-A-I-G-E and M-A-T-T. a. Draw the structure of each peptide at a pH of 7.0 b. Draw the structure of each peptide at a pH of 12.0 c. Calculate the pl of both peptides. Based on your answer, do you think these two peptides can be separated using ion exchange chromatography at a pH of 5? Explain.
1. Consider the peptide with amino acid sequence NICE. a. Write the full name and the three-letter abbreviations of the amino acids in the реptide. b. Calculate the net charge of the peptide at pH 7.0. Show you work in a table. с. Calculate the pI of the peptide. Show you work in a table.
The amino acid sequence of three peptide fragments are shown below. Peptide 1: QAMGRAGDLKYLGLHSV Peptide 2: ALMALFMVMALVLVSVLFIA Peptide 3: MVEDLLKQIARYLISE (a) Circle all of the charged residues in peptide 1 (assume pH =7.0). Box all of the aromatic residues in peptide 2. Underline all of the nonpolar residues in peptide 3. (b) Determine the net charge of the predominant form of each of the peptides at pH 4.5 and pH 11.5. Assume the ionizable groups have the pKa values listen in Table 2.1 of your text. (c) Which of these peptides would be most likely to be found as an alpha helix in a soluble (cytoplasmic) protein? Which would be most likely to be found as an alpha helix in a transmembrane protein? Which would be least likely to form an alpha helix of any kind?
4. a. Give the full names of the amino acids in this peptide. НзN — CH—С-N—CH— С-N—CH—С—О ČH3 H ČH,OH H. CH2 -ОН b. Calculate the isoelectric point (pl) of a peptide with sequence SNARE. Show your work in a table, giving the pKa values of the ionizable groups, pH, and charges. Step marks are counted. Don't need to write “protonated" or “deprotonated".
1. Consider the peptide with amino acid sequence NICE. а. Write the full name and the three-letter abbreviations of the amino acids in the peptide. b. Calculate the net charge of the peptide at pH 7.0. Show you work in a table. Step marks are counted. с. Calculate the pI of the peptide. Show you work in a table. Step marks are counted.
Consider the following peptide: alanyl-glutamyl-lysyl-cysteyl-asparagine. 1- Write the semi-developed formula of this peptide at pH = 1. Indicate the N- terminal and C-terminal ends of the peptide. Indicate the carbones a. 2- This peptide comprises 5 ionisable groups whose pKa values are, respectively: 2 ; 4.3 ; 8.3 ; 9.7 ; 10.5. Attribute a pKa value to each ionisable group. Justify your answer answer (using the Table, p. 8 when necessary).
The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has a pKa of 4.5. What is the net charge of this peptide at a pH of 1? Type your answer... ints The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has a pKa of 4.5. What is the net charge of this peptide at a pH of 7? Type your answer... The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has a pKa of 4.5. What is the net charge of this peptide at a pH of 15? Type your answer... The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has a pKa of 4.5. What is the isoelectric point of this peptide? Type your answer... Submit
The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has a pKa of 4.5. What is the net charge of this peptide at a pH of 1? Type your answer... ints The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has a pKa of 4.5. What is the net charge of this peptide at a pH of 7? Type your answer... 14- The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has a pKa of 4.5. What is the isoelectric point of this peptide? Type your answer... -- - The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has a pKa of 4.5. What is the net charge of this peptide at a pH of 15? Type your answer... --D Submit
1) Indicate the total charge of the peptide at pH 7.0, 9.0, and 11.0 2) Determine the isoelectric point (pI) for this peptide. Show the relevant pKa’s and your calculation. 3) What is the fraction of the peptide that has the N-terminal amino group deprotonated at pH 9.
4. A. Draw the structure of the following peptide sequence at pH 2.0: ASP-ALA-THR-LYS-GLY B. What is the net charge of this pentapeptide at pH 2.0?
HENRY is a peptide sequence. 1) Draw the structure of the HENRY peptide as it exists at pH 7. Be sure to label any and all charges. You can neglect the stereochemistry at the α carbon. 2) Now consider the longer peptide HENRYLINK. What is its charge at pH 7 and how does this charge compare to the charge of the HENRY peptide at pH 7? 3) Finally, draw a helical wheel diagram for the HENRYLINK peptide. How many turns of an α-helix does it encompass? Is the peptide amphipathic? Explain why or why not in a few sentences.
Refer to following peptide structure: H&N CH3 IZ CH3- CH₂OH IZ OH ZI CH3 CH3. OH IZ OH IZ CH2 CONH2 parce 0 IZ Calculate the net charge of this peptide at pH = 12. OH CH3 What is the identity of the second residue in the peptide shown below? Enter the capitalized single letter abbreviation. 0- Estimate the pl and round to the nearest whole number. Use pKa values provided in lecture.

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