a. What is the charge of the primary structure of proteins at pH = 2.0, 4.0, 6.0 and 11.0 of the following proteins. Draw their structures and predict their net charge. i.) Lys- Gly- Ala- Gly ii.) Lys- Gly-Ala-Glu iii.) His- Gly- Ala- Glu iv.) Glu- Gly- Ala -Glu v.) Gln-Gly-Ala-Lys
Before high performance liquid chromatography (HPLC) methods were devised for the separation and analysis of small peptides, electrophoresis on a paper support was frequently used. Separation was affected on the basis of the charge on a peptide at different pH values.
a. What is the charge of the primary structure of proteins at pH = 2.0, 4.0, 6.0 and 11.0 of the following proteins. Draw their structures and predict their net charge.
i.) Lys- Gly- Ala- Gly
ii.) Lys- Gly-Ala-Glu
iii.) His- Gly- Ala- Glu
iv.) Glu- Gly- Ala -Glu
v.) Gln-Gly-Ala-Lys
b.) Calculate the pI of the following primary structure of protein
c.) the migration toward the cathode, the negative pole; A for the migration toward the anode,
the positive pole; and O if the peptide remains stationary.
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