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4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are 45 kDa and the star shaped group is 27 kDa. The lines represent disulfide bonds.

4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are 45 kDa and the star shaped group is 27 kDa. The lines represent disulfide bonds.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are 45 kDa and the star shaped group is 27 kDa. The lines represent disulfide bonds. A. What is the size of the native protein? B. Draw an SDS-PAGE gel for the sample under reducing and non- reducing conditions. Clearly label the anode and cathode and label the lane that contains the reducing sample and the nonreducing sample. C. Name all of the noncovalent interactions that can occur in holding the star shaped monomer and the spiky monomers together. D. What is the most important interaction that holds monomers together in the native structure above?
Transcribed Image Text:4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are 45 kDa and the star shaped group is 27 kDa. The lines represent disulfide bonds. A. What is the size of the native protein? B. Draw an SDS-PAGE gel for the sample under reducing and non- reducing conditions. Clearly label the anode and cathode and label the lane that contains the reducing sample and the nonreducing sample. C. Name all of the noncovalent interactions that can occur in holding the star shaped monomer and the spiky monomers together. D. What is the most important interaction that holds monomers together in the native structure above?
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