6. In the citric acid cycle the enzyme aconitase catalyzes the conversion of citrate to isocitrate. The reaction is a dehydration-rehydration reaction occurring in two steps thru the intermediate aconitate. citrate s aconitate s isocitrate. When the enzyme aconitase is tested in the lab under standard conditions and the reaction allowed to run to equilibrium the concentration of citrate is 0.906 mM, aconitate 0.038 mM, and isocitrate 0.056 mM. a) isocitrate by the aconitase enzyme? Show calculations. (Standards and equations below) What is the AG" (standard free-energy) change for the conversion of citrate to R= 0.008315 kJ/mol·K T= 298 K (at 25°C) AGº=-RTIN Keq From standard conditions? your calculations explain whether the citric acid cycle could proceed under From the AG° calculations you made for the reactions catalyzed by aconitase, thermodynamically how does the citric acid cycle proceed under cellular conditions?
Electron Transport Chain
The electron transport chain, also known as the electron transport system, is a group of proteins that transfer electrons through a membrane within mitochondria to create a gradient of protons that drives adenosine triphosphate (ATP)synthesis. The cell uses ATP as an energy source for metabolic processes and cellular functions. ETC involves series of reactions that convert redox energy from NADH (nicotinamide adenine dinucleotide (NAD) + hydrogen (H)) and FADH2(flavin adenine dinucleotide (FAD)) oxidation into proton-motive force(PMF), which is then used to synthesize ATP through conformational changes in the ATP synthase complex, a process known as oxidative phosphorylation.
Metabolism
Picture a campfire. It keeps the body warm on a cold night and provides light. To ensure that the fire keeps burning, fuel needs to be added(pieces of wood in this case). When a small piece is added, the fire burns bright for a bit and then dies down unless more wood is added. But, if too many pieces are placed at a time, the fire escalates and burns for a longer time, without actually burning away all the pieces that have been added. Many of them, especially the larger chunks or damp pieces, remain unburnt.
Cellular Respiration
Cellular respiration is the cellular process involved in the generation of adenosine triphosphate (ATP) molecules from the organic nutritional source obtained from the diet. It is a universal process observed in all types of life forms. The glucose (chemical formula C6H12O6) molecules are the preferred raw material for cell respiration as it possesses a simple structure and is highly efficient in nature.
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