6. The enzyme prostaglandin endoperoxide synthase (PTGS) catalyzes the conversion of arachidonic acid to PGG₂, a precursor of prostaglandins. Ibuprofen is a potent inhibitor of PTGS. The following data were obtained for reactions catalyzed by PTGS in the absence and presence of ibuprofen ( [Arachidonic acid] (mm) 0.5 1 1.5 2.5 3.5 Rate of formation of PGG₂ (mm/min) 23.5 32.2 36.9 41.8 44.0 Rate of formation of PGG; with 10 mg/ml. ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 (a) Using Excel or an equivalent program, graph the data on a Lineweaver-Burk plot. Attach a graph of your work with properly labeled axes and equations for the linear regressions. (b) Determine the KM and Vmax of the reaction catalyzed by PTGS in the absence of ibuprofen as well as the apparent KM and apparent Vmax of the reaction in the presence of ibuprofen. (c) What type of inhibition does ibuprofen exert on PTGS? Briefly explain your answer.
6. The enzyme prostaglandin endoperoxide synthase (PTGS) catalyzes the conversion of arachidonic acid to PGG₂, a precursor of prostaglandins. Ibuprofen is a potent inhibitor of PTGS. The following data were obtained for reactions catalyzed by PTGS in the absence and presence of ibuprofen ( [Arachidonic acid] (mm) 0.5 1 1.5 2.5 3.5 Rate of formation of PGG₂ (mm/min) 23.5 32.2 36.9 41.8 44.0 Rate of formation of PGG; with 10 mg/ml. ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 (a) Using Excel or an equivalent program, graph the data on a Lineweaver-Burk plot. Attach a graph of your work with properly labeled axes and equations for the linear regressions. (b) Determine the KM and Vmax of the reaction catalyzed by PTGS in the absence of ibuprofen as well as the apparent KM and apparent Vmax of the reaction in the presence of ibuprofen. (c) What type of inhibition does ibuprofen exert on PTGS? Briefly explain your answer.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![6. The enzyme prostaglandin endoperoxide synthase (PTGS) catalyzes the conversion of arachidonic
acid to PGG2, a precursor of prostaglandins. Ibuprofen is a potent inhibitor of PTGS. The following
data were obtained for reactions catalyzed by PTGS in the absence and presence of ibuprofen (
[Arachidonic
acid] (mm)
0.5
1
1.5
2.5
3.5
Rate of formation of
PGG₂ (mm/min)
23.5
32.2
36.9
41.8
44.0
Rate of formation of PGG₂ with
10 mg/ml ibuprofen (mm/min)
16.67
25.25
30.49
37.04
38.91
(a) Using Excel or an equivalent program, graph the data on a Lineweaver-Burk plot. Attach a graph
of your work with properly labeled axes and equations for the linear regressions.
(b) Determine the KM and Vmax of the reaction catalyzed by PTGS in the absence of ibuprofen as well
as the apparent KM and apparent Vmax of the reaction in the presence of ibuprofen.
(c) What type of inhibition does ibuprofen exert on PTGS? Briefly explain your answer.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa6fdb913-5513-48f0-8c37-c1f95f49bc32%2F31e6350c-c711-4f00-ad78-16fcd401caf3%2F72m8csg_processed.jpeg&w=3840&q=75)
Transcribed Image Text:6. The enzyme prostaglandin endoperoxide synthase (PTGS) catalyzes the conversion of arachidonic
acid to PGG2, a precursor of prostaglandins. Ibuprofen is a potent inhibitor of PTGS. The following
data were obtained for reactions catalyzed by PTGS in the absence and presence of ibuprofen (
[Arachidonic
acid] (mm)
0.5
1
1.5
2.5
3.5
Rate of formation of
PGG₂ (mm/min)
23.5
32.2
36.9
41.8
44.0
Rate of formation of PGG₂ with
10 mg/ml ibuprofen (mm/min)
16.67
25.25
30.49
37.04
38.91
(a) Using Excel or an equivalent program, graph the data on a Lineweaver-Burk plot. Attach a graph
of your work with properly labeled axes and equations for the linear regressions.
(b) Determine the KM and Vmax of the reaction catalyzed by PTGS in the absence of ibuprofen as well
as the apparent KM and apparent Vmax of the reaction in the presence of ibuprofen.
(c) What type of inhibition does ibuprofen exert on PTGS? Briefly explain your answer.
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