5) You've extracted the total protein from the secretory glands of the venomous ferdelance snake (a South and Central American pit viper). The total volume of the crude extract was 3 ml, and a 10 microliter aliquot produced an absorbance of 0.75 absorbance units in a standard 2 ml assay. Using this information, and the standard curve below, estimate the total protein and protein concentration in the original crude extract. Abs 590 nm 1.2 1 0.8 0.6 0.4 0.2 Bradford Assay Standard Curve Series 1 0 0 20 40 60 80 100 120 micrograms of protein/ml
5) You've extracted the total protein from the secretory glands of the venomous ferdelance snake (a South and Central American pit viper). The total volume of the crude extract was 3 ml, and a 10 microliter aliquot produced an absorbance of 0.75 absorbance units in a standard 2 ml assay. Using this information, and the standard curve below, estimate the total protein and protein concentration in the original crude extract. Abs 590 nm 1.2 1 0.8 0.6 0.4 0.2 Bradford Assay Standard Curve Series 1 0 0 20 40 60 80 100 120 micrograms of protein/ml
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 24SST
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Transcribed Image Text:5) You've extracted the total protein from the secretory glands of the venomous ferdelance snake (a
South and Central American pit viper). The total volume of the crude extract was 3 ml, and a 10
microliter aliquot produced an absorbance of 0.75 absorbance units in a standard 2 ml assay. Using this
information, and the standard curve below, estimate the total protein and protein concentration in the
original crude extract.
Abs 590 nm
1.2
1
0.8
0.6
0.4
0.2
Bradford Assay Standard Curve
Series 1
0
0
20
40
60
80
100
120
micrograms of protein/ml
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