5) Under specific conditions, hemoglobin dissociates into its four subunits. The isolated a subunit binds oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen to the isolated a subunit is not affected by pH, or the allosteric regulator BPG. Use your knowledge about hemoglobin and the information provided in this question to answer: a. Draw the expected O₂ binding curves for tetrameric hemoglobin and for the isolated a subunit (draw them on the same axes). How can you explain their different behavior? b. Draw the expected Hill plot for tetrameric hemoglobin and for the isolated a subunit. c. What Hill coefficient (n) will you expect for tetrameric hemoglobin and for the isolated a subunit? d. How would you use each of these graphs to determine dissociation constants for the binding of oxygen to tetrameric hemoglobin and to the isolated a subunit? e. Why is the isolated a subunit unaffected by pH or BPG?
5) Under specific conditions, hemoglobin dissociates into its four subunits. The isolated a subunit binds oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen to the isolated a subunit is not affected by pH, or the allosteric regulator BPG. Use your knowledge about hemoglobin and the information provided in this question to answer: a. Draw the expected O₂ binding curves for tetrameric hemoglobin and for the isolated a subunit (draw them on the same axes). How can you explain their different behavior? b. Draw the expected Hill plot for tetrameric hemoglobin and for the isolated a subunit. c. What Hill coefficient (n) will you expect for tetrameric hemoglobin and for the isolated a subunit? d. How would you use each of these graphs to determine dissociation constants for the binding of oxygen to tetrameric hemoglobin and to the isolated a subunit? e. Why is the isolated a subunit unaffected by pH or BPG?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Please help me with 5 e) why is the isolated subunit unaffected by pH or BPG?
![5) Under specific conditions, hemoglobin dissociates into its four subunits. The isolated a subunit binds
oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen
to the isolated a subunit is not affected by pH, or the allosteric regulator BPG. Use your knowledge about
hemoglobin and the information provided in this question to answer:
a. Draw the expected O₂ binding curves for tetrameric hemoglobin and for the isolated a subunit
(draw them on the same axes). How can you explain their different behavior?
b. Draw the expected Hill plot for tetrameric hemoglobin and for the isolated a subunit.
c. What Hill coefficient (n) will you expect for tetrameric hemoglobin and for the isolated a subunit?
d. How would you use each of these graphs to determine dissociation constants for the binding of
oxygen to tetrameric hemoglobin and to the isolated a subunit?
e. Why is the isolated a subunit unaffected by pH or BPG?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe0719576-6288-4966-bcac-ea0ff9b5df4d%2F07b47b8e-8725-4984-946a-2b066a3c16e9%2Fft7qnsl_processed.jpeg&w=3840&q=75)
Transcribed Image Text:5) Under specific conditions, hemoglobin dissociates into its four subunits. The isolated a subunit binds
oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen
to the isolated a subunit is not affected by pH, or the allosteric regulator BPG. Use your knowledge about
hemoglobin and the information provided in this question to answer:
a. Draw the expected O₂ binding curves for tetrameric hemoglobin and for the isolated a subunit
(draw them on the same axes). How can you explain their different behavior?
b. Draw the expected Hill plot for tetrameric hemoglobin and for the isolated a subunit.
c. What Hill coefficient (n) will you expect for tetrameric hemoglobin and for the isolated a subunit?
d. How would you use each of these graphs to determine dissociation constants for the binding of
oxygen to tetrameric hemoglobin and to the isolated a subunit?
e. Why is the isolated a subunit unaffected by pH or BPG?
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