Given the following protein: N-MACHKGFDSTRRKYWQNKRLCVSA|IDWQSPWKNQGILV-C The overall charge of this protein at pH 7 is Positive In the first dimension of 2-D PAGE, this protein's isoelectric focusing point is between 8 and 10 * The average molecular weight of an amino acid is 113 daltons. The molecular weight of the protein above is daltons (don't round). A mole of the above protein weighs g. For 1 mL of a 4 mM solution of the above protein, you would need to weigh out (number and unit, at least one non-zero integer to the left of the decimal point, don't round)
Given the following protein: N-MACHKGFDSTRRKYWQNKRLCVSA|IDWQSPWKNQGILV-C The overall charge of this protein at pH 7 is Positive In the first dimension of 2-D PAGE, this protein's isoelectric focusing point is between 8 and 10 * The average molecular weight of an amino acid is 113 daltons. The molecular weight of the protein above is daltons (don't round). A mole of the above protein weighs g. For 1 mL of a 4 mM solution of the above protein, you would need to weigh out (number and unit, at least one non-zero integer to the left of the decimal point, don't round)
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter31: Completing The Protein Life Cycle: Folding, Processing, And Degradation
Section: Chapter Questions
Problem 20P
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Need help on questions 3, 4, and 5.

Transcribed Image Text:Given the following protein:
N-MACHKGFDSTRRKYWQNKRLCVSAI|DWQSPWKNQGILV-C
The overall charge of this protein at pH 7 is Positive
In the first dimension of 2-D PAGE, this protein's isoelectric focusing point is
between 8 and 10 +
The average molecular weight of an amino acid is 113 daltons. The molecular weight of
the protein above is
daltons (don't round).
A mole of the above protein weighs
g. For 1 mL of a 4 mM solution of the
above protein, you would need to weigh out
(number and unit,
at least one non-zero integer to the left of the decimal point, don't round)
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