A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to gelelectrophoresis in the presence of sodium dodecyl sulfate (SDS), the protein gives three bands with molecular masses of180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol (DTT), three bands againform, this time with molecular masses of 160, 90, and 60 kDa.How many subunits does the protein have, and what is the molecular mass of each?four subunits: 180, 160, 90, and 60 kDathree subunits: 180, 160, and 60 kDathree subunits: 160, 90, and 60 kDafour subunits: 160, 90, 90, and 60 kDaCorrect Answer

The key to solving this problem is comparing the molecular masses obtained under two different…

Answered: A protein has a molecular mass of 400…

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.1: Introduction To Clinical Chemistry

Problem 9RQ

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a. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Draw the pathway leading to the production of protein E.
b. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Denote the point in the pathway in which each individual is blocked.
In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaffs rule, reconstruct the missing data and list the base composition of the DNA sample.
What are Okazaki fragments and how they are formed?
Both of the regular intravenous solutions administered in medicine, normal saline and lactated Ringer’s solution, are isotonic. Why is this important?
Describe the differences in the four protein structures.
The synthetic process by which monomers are covalently linked is (a) hydrolysis (b) isomerization (c) condensation (d) glycosidic linkage (e) ester linkage
Polypeptide folding is often mediated by other proteins called chaperones. Describe how a mutant chaperone protein might be responsible for a genetic disorder involving an enzyme.
Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain surgical procedures, such as those for malignancy, the surgeon may request that sterile water be used for irrigation instead of saline following excision of the cancerous mass. Why is normal saline usually used in healthy tissues?

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