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5) The restriction enzyme EcoRI recognizes the DNA sequence GAATTC and makes a staggered cut as shown in the blue figure below: GA ATTC CTTAAG If EcoRI cuts the generic fragment shown below, 2 fragments with "sticky ends" (single-stranded DNA) will result. "XXX.* denotes any combination of nucleotides that flank either side of the sequence. 6 base pairs 8bp's 5'-CTGTAGAATICGTTACGA - 3' 3'-GACATCTTAAGCAATGCT- 5' AATTCGTTACGA GCAATGCT CTGTAG EcoRI Digest GACATCTTAA A. Given the sequence in the section of DNA shown below, draw boxes around the fragments that will be formed when cutting this region of DNA with EcoRI and HAEIII at the same time. 5'- GTGGCCATTCTAGCGCCGAATTCGGCAATTACGATCGTAGGCCATCGATGTAATTC -3' 3'- CACCGGTAAGATCGCGGCTTAAGCCGTTAATGCTAGCATCCGGTAGCTACATTAAG -5' B. Give the sizes in base pairs of each of the fragments that will be formed after making the cuts with these 2 enzymes (don't count the nucleotides in the single-stranded parts).

5) The restriction enzyme EcoRI recognizes the DNA sequence GAATTC and makes a staggered cut as shown in the blue figure below: GA ATTC CTTAAG If EcoRI cuts the generic fragment shown below, 2 fragments with "sticky ends" (single-stranded DNA) will result. "XXX.* denotes any combination of nucleotides that flank either side of the sequence. 6 base pairs 8bp's 5'-CTGTAGAATICGTTACGA - 3' 3'-GACATCTTAAGCAATGCT- 5' AATTCGTTACGA GCAATGCT CTGTAG EcoRI Digest GACATCTTAA A. Given the sequence in the section of DNA shown below, draw boxes around the fragments that will be formed when cutting this region of DNA with EcoRI and HAEIII at the same time. 5'- GTGGCCATTCTAGCGCCGAATTCGGCAATTACGATCGTAGGCCATCGATGTAATTC -3' 3'- CACCGGTAAGATCGCGGCTTAAGCCGTTAATGCTAGCATCCGGTAGCTACATTAAG -5' B. Give the sizes in base pairs of each of the fragments that will be formed after making the cuts with these 2 enzymes (don't count the nucleotides in the single-stranded parts).

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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5) The restriction enzyme EcoRI recognizes the DNA sequence GAATTC and makes a staggered cut as shown in the blue figure below: GA ATTC CTTAAG If EcoRI cuts the generic fragment shown below, 2 fragments with "sticky ends" (single-stranded DNA) will result. "XXX.." denotes any combination of nucleotides that flank either side of the sequence. 6 base pairs 8bp's 5-СTGTAGAATІCGTTACGA- 3' 3-GACATCTTAAGCAATGCT- 5 CTGTAG EcoRI Digest AATTCGTTACGA GCAATGCT GACATCTTAA A. Given the sequence in the section of DNA shown below, draw boxes around the fragments that will be formed when cutting this region of DNA with EcoRI and HAEIII at the same time. 5'- GTGGCCATTCTAGCGCCGAATTCGGCAATTACGATCGTAGGCCATCGATGTAATTC -3' 3'- CACCGGTAAGATCGCGGCTTAAGCCGTTAATGCTAGCATCCGGTAGCTACATTAAG -5' B. Give the sizes in base pairs of each of the fragments that will be formed after making the cuts with these 2 enzymes (don't count the nucleotides in the single-stranded parts).
Transcribed Image Text:5) The restriction enzyme EcoRI recognizes the DNA sequence GAATTC and makes a staggered cut as shown in the blue figure below: GA ATTC CTTAAG If EcoRI cuts the generic fragment shown below, 2 fragments with "sticky ends" (single-stranded DNA) will result. "XXX.." denotes any combination of nucleotides that flank either side of the sequence. 6 base pairs 8bp's 5-СTGTAGAATІCGTTACGA- 3' 3-GACATCTTAAGCAATGCT- 5 CTGTAG EcoRI Digest AATTCGTTACGA GCAATGCT GACATCTTAA A. Given the sequence in the section of DNA shown below, draw boxes around the fragments that will be formed when cutting this region of DNA with EcoRI and HAEIII at the same time. 5'- GTGGCCATTCTAGCGCCGAATTCGGCAATTACGATCGTAGGCCATCGATGTAATTC -3' 3'- CACCGGTAAGATCGCGGCTTAAGCCGTTAATGCTAGCATCCGGTAGCTACATTAAG -5' B. Give the sizes in base pairs of each of the fragments that will be formed after making the cuts with these 2 enzymes (don't count the nucleotides in the single-stranded parts).
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