47. Calculate [OH-] in each aqueous solution at 25 °C, and classify the solution as acidic or basic. MISSED THIS? Read Section 17.5 a. [H3O+] = 1.2 × 10-8 M c. [H3O+] = 3.5 x 10-2 M b. [H3O+] = 8.5 x 10-5 M
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- Tomato juice has a OH- concentration equal to 1 X 10 -10, what is the pH of this solution?Calculate the concentration of Y4-ion in 0.0100 M EDTA solution at pH 6. α4 = 2.2 x 10-5 for pH 6.The concentration of the conjugate base in 100 mL of 50 mM CH3COOH/CH3COO- (pKa = 4.76) at pH 5.00 is ______mM. If 100 μL of 1.0 M NaOH is added, the buffer pH is expected to shift to ______ please answer it and explain .
- The concentration of the conjugate base in 100 mL of 50 mM CH3COOH/CH3COO- (pKa = 4.76) at pH 5.00 is ______mM. If 100 μL of 1.0 M NaOH is added, the buffer pH is expected to shift to_______ .You need a buff er at pH 7.5 for use in purifying a protein at 4°C. You have chosen Tris, pK 8.08, ΔH° = 50 kJ · mol−1. You carefully make up 0.01 M Tris buffer, pH 7.5 at 25°C, and store it in the cold to equilibrate it to the temperature of the purifi cation. When you measure the pH of the temperature-equilibrated buff er it has increased to 8.1. What is the explanation for this increase? How can you avoid this problem?47)
- (please type answer no write by hand)Draw the titration curve for an 800 mL 0.25 M solution of Arginine. (Graph pH vs. mole OH-.) Where onthe curve is the charge on Arginine +1.5? Identify it on the graph.Quinine ( C20 H24 N2 O2) is the most important alkaloid derived from cinchona bark. It is used as an antimalarial drug. For quinine, pK, = 5.1 and pK, = 9.7 ( pKp = – log Kp). Only 1 g quinine will dissolve in 1920.0 mL of solution. Calculate the pH of a saturated aqueous solution of quinine. Consider only the reaction | Q+ H2O= QH+ + OH- described by pK, where Q = quinine. pH =
- Calculate the concentration of Y4-ion in 0.0100 M EDTA solution at pH 6. for pH 6, α4 = 2,2 x 10-5An enzyme (molecular weight= 24 kDa, pI= 5.5) is contaminated with two other proteins, one witha similar molecular mass and a pI of 7.0 while the other has a molecular mass of 100 kDa and a pIof 5.4. Suggest how the contaminated enzyme can be purified.Describe how you would prepare a 500 ml of 0.35M Glycine pH2.2 from a 5M Glycine solution of pH 7. Show your calculations.