3. For each of the following characteristics, list all of the bases (A, B, C, or D) to which they apply. (a) A signal that identifies a parental DNA strand in the MutH,L,S mismatch correction system (b) Most likely to be involved in cyclobutane dimer formation after A B D HN-CH3 o-CH3 NH2 CH3 CH3 HN HN ultraviolet irradiation of DNA H,N (c) A methylated base found immediately to the 5' side of dGMP residues in eukaryotic DNA (d) Created by treating DNA with alkylating agents that transfer methyl groups and repaired by an "enzyme" that functions only once in its lifetime (e) Created by AdoMet-dependent methylation of a nucleotide residue in DNA (f) A substrate for deamination at the DNA level, which would lead to a GC→ AT transition

3. For each of the following characteristics, list all of the bases (A, B, C, or D) to which they apply. (a) A signal that identifies a parental DNA strand in the MutH,L,S mismatch correction system (b) Most likely to be involved in cyclobutane dimer formation after A B D HN-CH3 o-CH3 NH2 CH3 CH3 HN HN ultraviolet irradiation of DNA H,N (c) A methylated base found immediately to the 5' side of dGMP residues in eukaryotic DNA (d) Created by treating DNA with alkylating agents that transfer methyl groups and repaired by an "enzyme" that functions only once in its lifetime (e) Created by AdoMet-dependent methylation of a nucleotide residue in DNA (f) A substrate for deamination at the DNA level, which would lead to a GC→ AT transition

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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18. If 32P is used to label both strands of double-stranded DNA, after two rounds of replication, four double-stranded molecules of DNA are produced. Of these,a. one of the four double-stranded molecules of DNA will contain all the 32Pb. two of the four double-stranded molecules of DNA will contain 32P, each with half of the 32Pc. three of the four double-stranded molecules of DNA will contain 32P, each with 1/3 of the 32Pd. all four double-stranded molecules of DNA will contain 32P, each with ¼ of the 32Pe. none of the double-stranded molecules of DNA will contain 32P
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1. Why were nuclei being unstained when you used eosin? 2. What is the relationship between heterochromatin and the synthetic activity of DNA? 3. What is the significance of using different histological stains? 4. Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and nucleic acid? What are the differences? 5. How essential is Periodic Acid- Schiff Reagent? please answer with 3-5 sentences
3) Why are single strand binding proteins (ssb proteins) needed in DNA replication? (note that you need to explain WHY ssb proteins are important, not just what they do)
3. A long DNA will be divided into small fragments, and one of them will be attached to the bead and amplified in Ion Torrent sequencing. The beads with different kinds of fragments will be loaded into different wells. The following graphs show the first forty (40) signals from two different wells. The value of one (1) in the y-axis of the graph corresponds to incorporation of one (1) nucleotide to every DNA strand on a bead. (a) You can observe that in every single step the signals are not the multiples of an integer. Explain why such signals are produced and how we can interpret the signals. (b) Using the first forty (40) signals shown below, please figure out the DNA sequence of the fragment attached to the bead in each well. (c) Based on the sequences of the fragments, can we guess the partial sequence of the original DNA sequence (before fragmentation)? If so, what could it be? (d) In the well #1, when the non-zero signals will show up after the 40 steps? Answer this by the step…
1) Using the diagram below, sketch in the pattern of bands you would expect to see after digesting the DNA of the TT, Tt and tt genotypes of the TAS2R38 gene. Use the Base Pair Standards on the left of the diagram as a reference in drawing the positions of the bands. Base Pair TASTER GENO TYPE Standards (Base Pairs) TT Tt tt 500 400 300 200 100 50
8
1. What structures are these DNA strand likely to adopt in solution (assuming sufficient salt concentration to permit any hybridisation as appropriate)? Draw your answers depicting the hybridisation. a) GCC TTG AGC TTT TTT GCT CAA GGC (b) ATG ACT CTC GAG AGT CAT TTA TTA
7. F. State if/how Remedios' mutation changes the amino acid sequence and describe the effect that change has, if any, on the protein.
Please answer all the question. I dont need the explanations. Thanks!
3. In the following drawing, the top strand is the template DNA, and the bottom strand shows the lagging strand prior to the action of DNA Polymerase I. Three Okazaki fragments are shown and the RNA primers (asterisks) have not yet been removed. 3'- -5' 5********** RNA primer Left Okazaki fragment ↑ RNA primer ↑ -||- Middle Okazaki fragment -||- *********** RNA primer Right Okazaki fragment -3' A. Which Okazaki fragment was made first, the one on the left or the one on the right? B. Which RNA primer would be the first one to be removed by DNA polymerase I, the primer on the left or the primer on the right? For this primer to be removed by DNA polymerase I and for the gap to be filled in, is it necessary for the Okazaki fragment in the middle to have already been synthesized? Explain why. C. Let's consider how DNA ligase connects the left Okazaki fragment with the middle Okazaki fragment. After DNA polymerase I removes the middle RNA primer and fills in the gap with DNA, where does DNA…
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