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- 3. Identify C-Cl stretching peaks in CDC13 and CHC13 spectra. Do these peaks have exactly the same frequencies for both compounds? Explain your answer. 4. Is the C==O stretching frequency the same for acetone and deuterated acetone? Explain your answer. 5. Identify C==O overtone in acetone spectrum which corresponds to the transition from ground level, n=0, to the second excited level, n=2.What are T1 and T2 relaxation times in NMR spectrometry? How are pulse sequences used in NMR?Why does Cytochrome C have a peak absorbance of 409nm? When proteins usually have a peak absorbance of 280nm..?
- 1. Answer BOTH parts a) and b). a) i) Explain the difference between spin-lattice relaxation (T₁) and spin-spin relaxation (T2). ii) Deuterated acetone, (CD3)2CO, is a common solvent for NMR spectroscopy. Sketch the 1³C{¹H} NMR spectrum of this solvent. Label and explain any coupling in your spectrum. iii) Predict the structure of the residual protio-solvent species observed in the ¹H NMR spectrum of (CD3)2CO. b) OH A B C max 260 nm 295 nm i) Suggest electronic transitions that can be attributed to the absorptions reported for compounds A and B, respectively. ii) With the aid of diagrams explain why B absorbs at a lower energy to A. iii) Explain why compound C possesses a charge transfer transition. iv) For compound C explain why changing solvent polarity influences the wavelength of the charge transfer absorption. -CEZ4. Why does a carbon to oxygen double bond absorption band have a greater intensity than a carbon to carbon double bond absorption band?What type(s) of molecular motion is (are) observed using infrared spectroscopy? A. Stretching and bending B. Rotation and excitation C. Spin flipping. D. Fragmentation
- Starting material was [(Me5dien)CoCl2] and reacted with SCN to produce [(Me5dien)Co(SCN)2]. Using the two graphs given below how does the shifts in lamdamax for the observed peaks support your notion that Cl has been replaced with N? [(Me5dien)CoCl2] Peak A: 531.8 nm, 0.722 Abs Peak B: 609.7 nm, 0.666 Abs [(Me5dien)Co(SCN)2] Peak A: 510.2 nm, 0.357 Abs Peak B: 608.10 nm, 0.401 AbsHow do the 1D and 2D or 3D NMR Spectroscopy differ from detection and analysis perspectives?5. A sample of a red dye and a sample of a blue dye both have the same concentration. Their Absorbance is measured using the same spectrophotometer. At a particular wavelength, will both samples give the same Absorbance reading? Why or why not?
