2. Calculate the effective dilution. You were given a sample which contained 5.5mL of bacterial culture. You added 10uL of phage solution to the culture. What was the effective dilution produced by the addition of the phage solution?
2. Calculate the effective dilution. You were given a sample which contained 5.5mL of bacterial culture. You added 10uL of phage solution to the culture. What was the effective dilution produced by the addition of the phage solution?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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![### Problem Statement
**Calculate the Effective Dilution:**
You were given a sample that contained 5.5 mL of bacterial culture. You added 10 μL of phage solution to the culture. What was the effective dilution produced by the addition of the phage solution?
### Explanation
To determine the effective dilution, you need to calculate the total volume and then find the ratio of the original phage solution volume to this total volume.
1. **Volume of Bacterial Culture:** 5.5 mL
2. **Volume of Phage Solution Added:** 10 μL (convert to mL: 0.01 mL)
**Total Volume:**
\[
5.5 \, \text{mL} + 0.01 \, \text{mL} = 5.51 \, \text{mL}
\]
**Effective Dilution Formula:**
\[
\text{Dilution} = \frac{\text{Volume of Phage Solution}}{\text{Total Volume}} = \frac{0.01}{5.51}
\]
**Calculation:**
\[
\text{Effective Dilution} = \frac{0.01}{5.51} \approx 0.001815 \, \text{(1 in 551)}
\]
Therefore, the effective dilution of the phage solution is approximately 1:551.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F86b8ecca-a53c-43d3-8cd5-fb975ff4ee22%2F7fe54626-3e6b-4c60-acfb-cc3d43f4b472%2Fsunkq3m_processed.jpeg&w=3840&q=75)
Transcribed Image Text:### Problem Statement
**Calculate the Effective Dilution:**
You were given a sample that contained 5.5 mL of bacterial culture. You added 10 μL of phage solution to the culture. What was the effective dilution produced by the addition of the phage solution?
### Explanation
To determine the effective dilution, you need to calculate the total volume and then find the ratio of the original phage solution volume to this total volume.
1. **Volume of Bacterial Culture:** 5.5 mL
2. **Volume of Phage Solution Added:** 10 μL (convert to mL: 0.01 mL)
**Total Volume:**
\[
5.5 \, \text{mL} + 0.01 \, \text{mL} = 5.51 \, \text{mL}
\]
**Effective Dilution Formula:**
\[
\text{Dilution} = \frac{\text{Volume of Phage Solution}}{\text{Total Volume}} = \frac{0.01}{5.51}
\]
**Calculation:**
\[
\text{Effective Dilution} = \frac{0.01}{5.51} \approx 0.001815 \, \text{(1 in 551)}
\]
Therefore, the effective dilution of the phage solution is approximately 1:551.
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