You are using the bacterium E. coli to synthesize a protein from recombinant DNA. The protein remainsin the bacterium, so you must lyse the cell in order obtain the protein.1. Describe very, very briefly (i. e. short sentences) the steps you will take to disrupt the microbe.

Answered: You are using the bacterium E. coli to…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You are using the bacterium E. coli to synthesize a protein from recombinant DNA. The protein remains
in the bacterium, so you must lyse the cell in order obtain the protein.
1. Describe very, very briefly (i. e. short sentences) the steps you will take to disrupt the microbe.

Expert Solution

Step 1

Microorganisms incorporate every single unicellular living being as are very assorted. Of the three spaces of life distinguished via Carl Woese, the entirety of the Archaea and Bacteria are microorganisms. These were recently assembled in the two space framework as Prokaryotes, the other being the eukaryotes. The third area Eukaryota incorporates every single multicellular life form and numerous unicellular protists and protozoans. A few protists are identified with creatures and some to green plants. A large number of the multicellular life forms are tiny, specifically miniature creatures, a few organisms, and some green growth, yet these are not examined here.

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