1a-Consider a situation where the primer sequences used in a PCR reaction are synthesized to be complementary to a mutation in the human genome that leads to a genetic disorder. Which of the following statements is true? a- The PCR reaction will work if you use template DNA from someone who has the disorder. b- The PCR reaction will work if you use template DNA from someone who does not have the disorder. 1b- In the diagram attached, can the jellyfish DNA be directly treated with DNA ligase so that its genome is reassembled and lacks the GFP gene? a- No b- Yes
1a-Consider a situation where the primer sequences used in a PCR reaction are synthesized to be complementary to a mutation in the human genome that leads to a genetic disorder. Which of the following statements is true? a- The PCR reaction will work if you use template DNA from someone who has the disorder. b- The PCR reaction will work if you use template DNA from someone who does not have the disorder. 1b- In the diagram attached, can the jellyfish DNA be directly treated with DNA ligase so that its genome is reassembled and lacks the GFP gene? a- No b- Yes
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1a-Consider a situation where the primer sequences used in a PCR reaction are synthesized to be complementary to a mutation in the human genome that leads to a genetic disorder. Which of the following statements is true?
a- The PCR reaction will work if you use template DNA from someone who has the disorder.
b- The PCR reaction will work if you use template DNA from someone who does not have the disorder.
1b- In the diagram attached, can the jellyfish DNA be directly treated with DNA ligase so that its genome is reassembled and lacks the GFP gene?
a- No
b- Yes
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