10. What is the Rf value for Spot A on the TLC plate shown? solvent front Spot A 0 scale for measuring distances Jug GA A Solvent left in the sample can lower broaden the mp range distanet travel by cmpd original spot. locations 2 RF = distance travel by solvent Front 10 Rf= 11. Which of the following compounds will have a smaller Rf value on a TLC plate coated with silica gel and eluted with hexanes: ethyl acetate = 80:20 mixture? Circle it. (nonpolar)

10. What is the Rf value for Spot A on the TLC plate shown? solvent front Spot A 0 scale for measuring distances Jug GA A Solvent left in the sample can lower broaden the mp range distanet travel by cmpd original spot. locations 2 RF = distance travel by solvent Front 10 Rf= 11. Which of the following compounds will have a smaller Rf value on a TLC plate coated with silica gel and eluted with hexanes: ethyl acetate = 80:20 mixture? Circle it. (nonpolar)

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter13: An Introduction To Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 13.21QAP

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Similar questions

During TLC, what is the stationary phase and what is the mobile phase?
As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True False
In TLC, why does the developing solvent 3:1:1 n-butanol/acetic acid/water climb up the TLC plate so slowly?
1a) Hexane, CH3(CH2)4CH3, is found to have a long retention time when separated from a mixture. A column with a C18 (18-carbon chain) stationary phase and a methanol-water modal phase is used for the separation. Explain why hexane has a long retention time. 1b)You wish to chromatographically seperate a mixtureof the follwing compunds. Which of the two compounds will be most difficult to seperatate from one another? Why?
Silica gel is very polar stationary phase. Therefore, more polar molecule sticks to silica gel on the TLC plate and has higherRfvalue. True False
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hi, i need help on this one. It was rejected but i really need help on it! Thank you
Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.
Record your absorbance data for the YELLOW and RED dyes below. Calculate the dye concentration in mM for each cuvette using M1V2 = M2v2 . Remember the concentration of stock solutions (100^ % dye) is 0.020 mM(mM= millimolar =mm /L). Data for YELLOW: peak absorbance used on spectrophotometer =420nm Cuvette 1 - 100% dye = 0.680 absorption Cuvette 2 - 75% dye = 0.510 absorption Cuvette 3 - 50% dye = 0.340 absorption Cuvette 4 - 25% dye = 0.170 absorption Data for RED - peak absorption used on spectrophotometer = 500nm Cuvette 1 - 100% dye: 0.621 absorption Cuvette 2 - 75%: 0.466 absorption Cuvette 3 - 50% dye: 0.311 absorption Cuvette 4 - 25% dye: 0.1555 absorption
Explain why TLC cannot be used for some volatile low molecular weight substances such as toluene.
how does TLC determine if a sample is pure?
A chemist was able to extract a biologically active compound and wants to purify the sample since he is sure that the sample still contains impurities. After running the extract in TLC, the chemist found out that the sample produced only one spot near the solvent front. Suggest how the chemist can achieve a better resolution for the chromatogram.