A chemist was able to extract a biologically active compound and wants to purify the sample since he is sure that the sample still contains impurities. After running the extract in TLC, the chemist found out that the sample produced only one spot near the solvent front. Suggest how the chemist can achieve a better resolution for the chromatogram.

Q: What Are the Main Differences Between High Performance Liquid Chromatography and Gas Chromatography?

A: The high performance liquid chromatography and gas chromatography are used to determine the…

Q: In lon Chromatography retention times and the resolution between adjacent peaks can be affected by…

A: This concept is based on the properties of solutions, where the solute (in this case, ions) is…

Q: A student analyzes sample 1, and sample 2 chromatograms of a mixture containing two compounds (A +…

A: The two samples( Compound A+Compound B ) are undergone chromatography : Compound A and Compound B

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A:

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A: Here VT = 30 mL, Vs = 15 cm3 , and VL = 15 mL,

Q: What are the solid phases will you use in Column Chromatography?

A: Step 1:

Q: Describe how to obtain a Retention Factor. What does the retention factor tell you?

A: Retention factor of a material is the ratio of the distance the spot moved above the origin to the…

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Q: Are we able to completely separate pigments from each other using paper chromatography?

A: Paper chromatography is a type of chromatography in which a stationary phase, such as paper sheets…

Q: A student places an unknown sample on a thin-layer chromatography plate and develops it in…

A: The important points in the question are: An unknown sample was placed on a thin-layer…

Q: A student researcher performed a chromatographic separation of caffeine and aspartame. The retention…

A: Given tc = 209.6 sωc = 14.4 sta = 260.0 sωa = 21.2 sd = 22.1 s

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A: Paper chromatography is the chromatography based on liquid-liquid partition chromatography. It…

Q: stationary phase and mobile phase

A:

Q: trying benzophenone using column chromatography. They have prepared a mixture of standard…

A:

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A: To find out the time that components A and B spend in the stationary phase.  The chromatogram given…

Q: What is the %composition of the Vitamin A peak in the sample?

A: To determine the % composition of the Vitamin A peak in the sample.

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Q: How can TLC be used to determine if a sample is pure?

A: Thin Layer Chromatography (TLC) is a separation procedure that requires a very small sample. It is…