Answered: how does TLC determine if a sample is…

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter27: Gas Chromatography

Section: Chapter Questions

Problem 27.19QAP

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Similar questions

How can TLC be used to determine if a sample is pure?
How is TLC used to determine the purity of a sample?
During TLC, what is the stationary phase and what is the mobile phase?
what chromatography seperation how is tlc part of it
A student performs a TLC experiment and at the end of the experiment, sees no spots other than the initial spot. What does this mean, and what should the student do to fix the problem?
Although TLC is qualitative and tells you what is present in a given sample, it can also be used to determine where a compound might be lost: What samples could you test on a single TLC plate that might give you this information?
1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?
The following are the uses of TLC except Choices: Determine the number of components in a sample Detect a given compound or compounds in a very crude mixture Determine the concentration of certain components of the mixture Find suitable conditions prior to running a column chromatogram None of the above
Explain why TLC cannot be used for some volatile low molecular weight substances such as toluene.
PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.
Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.
Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC. -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.

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