1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. a. Pscl& Gsul b. Scal, Pdml & BsaXI c. Scal, Sspl. & Ebel

Q: Describe in detail how blue/white screening works in bacteria. As part of your description explain…

A: The blue-white screen is the screening technique by which the recombinant bacteria is detected…

Q: What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of…

A: Restriction fragment length polymorphism (RFLP) : it is a method used in genome mapping and…

Q: Plasmid is  3.6kb large. It is digested with an enzyme that has restriction sites at  1,152 and…

A: Plasmids are circular DNA found in bacteria. They are extra chromosomal materials and are highly…

Q: ased on the knowledge you gained from the cloning module, which of the lanes in the urs is…

A: Agarose gel electrophoresis is used to separate DNA molecules based on their size and analyze DNA…

Q: etion enzyme Sall cuts the sequence GTCGAC to leave a five base, 5' overhang. The restriction enzyme…

A: A restriction enzyme is classified under the category of a protein. These are isolated from bacteria…

Q: 83. A plasmid is cut with the restriction enzyme BamH1 giving fragments of 3000 and 1000 bp as…

A: In this plasmid, we got 2 fragments of length 1000 and 3000 bp after digestion with BamH1 that…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the  plasmid…

A: Deoxyribonucleic acid ( DNA) is the molecule that carries genetic information for an organism.The…

Q: 5. You wish to map restriction sites in an unknown plasmid that has not been sequenced. You digest…

A: The process of fragmenting deoxyribonucleic acid strands with specific enzymes is restriction…

Q: 18. An instructor had her students perform this laboratory beginning with setting up their own…

A: Agarose gel electrophoresis is the technique for the separation of DNA fragments on the basis of…

Q: This plasmid is digested with the enzymes listed below. Indicate how many fragments will be…

A:

Q: 5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL…

A: Introduction :- A plasmid is a small, circular, double-stranded DNA molecule that can replicate…

Q: arac PKAN-R 5,512 bp PARA 4,872 bp BamH PBAD Bam 377 bp kank ampR PBAD-rfp 807 bp Hindill Hindli

A: Answer :  The ristricton enzmye used to make cut in the plasmid.  Both the plasmid should be cut…

Q: 4. Animation 12.4: This table shows results from a genetic test conducted on DNA samples from two…

A: Restriction enzymes are the enzymes capable of cutting double-helical segments that contain a…

Q: Draw a diagram showing what pGEM will look like after it has been digested with BamHI.

A: BamHI: BamHI is a restriction enzyme obtained from Bacillus amyloliquefaciens that specifically cuts…

Q: When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with…

A: A plasmid is a double-stranded DNA molecule that is small and circular in shape.  Bactria and yeast…

Q: Which of the following would allow for control of a gene's orientation when inserted into a plasmid…

A: The technique of molecular biology used to generate similar copies of a particular segment (gene) of…

Q: 1. Given the following restriction endonucleases and the sequences of their corresponding…

A: Prokaryotic cells are unicellular organisms that differ from multicellular organisms in composition…

Q: dont provide handwriting solution ...

A: When dealing with circular DNA containing multiple EcoRI recognition sites, the digestion pattern…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Restriction mapping involves physically cuttings double stranded DNA by using restriction…

Q: How many DNA fragments will result when the double-stranded DNA molecule below is digested with…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: 25. The restriction enzymes Kpnl and Acc65l recognize and cleave the same 6-bp sequence. You have a…

A: Restriction enzymes are the DNA digesting enzymes.

Q: Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is…

A: Plasmid:Circular mostlySize of plasmid is between <5kb - >200kbMay or may not be present in…

Q: Many studies have linked plasmids to bacterial resistance to antibiotics.  Closured circular…

A: Focusing on plasmids as a target to combat antibiotic resistance holds promise but requires a…

Q: Pictured below is a map of the pBR322 plasmid vector. If a DNA fragment is inserted into the Sal I…

A: As Sal1 (also BamH1) codes for tetracycline resistance. Therefore, the insertion of DNA fragment…

Q: Match the features of plasmids below with their meaning : Origin of replication LacZ gene antibiotic…

A: Plasmid is rounded Structure in the bacteria apart from main genome which functions as a a vehicle…

Q: Margaret has been given a plasmid containing her favorite gene, afg (Margaret’s favorite gene). The…

A: First, convert the amount of plasmid from micrograms (μg) to nanograms (ng) since the concentration…

Q: Consider the following:  Restriction enzymes bind and cut between bases T and A.  Based on this,…

A: VNTR  stands for Variable Number of Tandem Repeats, means the DNA sequence (Satellite DNA) repeats…

Q: true or false if a restriction enzyme recognizes the restriction site, 5' AACGTT3', and the enzyme…

A: 1. Recognition Site of the Restriction Enzyme:The recognition site of a restriction enzyme is the…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Removing RNA is one of the crucial processes in plasmid purification, especially after the manual…

Q: If a F plasmid does not have the gene encoding TraJ, this F plasmid would

A: Conjugation is a major mechanism for dissemination of antibiotic resistance and virulence among…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.