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1. What color change do you observe in Part B of the experiment? In which of the test tubes did hydrolysis of protein take place? 2. In which of the test tubes would you say hydrolysis has taken place in Part C? Why?

1. What color change do you observe in Part B of the experiment? In which of the test tubes did hydrolysis of protein take place? 2. In which of the test tubes would you say hydrolysis has taken place in Part C? Why?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Answer the questions.

1. What color change do you observe in Part B of the experiment? In which of the test tubes did hydrolysis of protein take place?

2. In which of the test tubes would you say hydrolysis has taken place in Part C? Why?

 

Based your answers in the attached procedure. 

 

 

B. Action of Pancreatic Proteases and Trypsin on Protein 1. Prepare 3 test tubes containing 2 mL egg albumin solution. Tube 1: Add 1 mL 5% Trypsin Tube 2: Add 1 mL 5% Trypsin and 2 mL of 0.5% Na₂CO3 Solution Tube 3: Add 2 mL 0.5% Na₂CO3 Solution 2. Place all test tubes in hot water bath maintained at 38-40°C for one hour. 3. Filter the contents of each test tube and use the filtrate for biuret test. C. Action of Bromelain in the Setting of Gelatin 1. Label 5 test tubes from 1 to 5. Place the substances as indicated in the table below. Test Tube 1 (Control) 2 3 4 5 Gelatin 2 ml 2 ml 2 ml 2 ml Pineapple Juice 1 ml (boiled) 1 ml 1 ml Meat Tenderizer 0.25M MgCl2 5 drops 1 ml 1 ml Water 2 ml 5 mg a. A purified preparation of papain may be used instead of the meat tenderizer. b. Test tube 2 should be prepared by putting 1 ml of pineapple juice in the test tube and placing it in a boiling water bath for 5 minutes. The tubes should then be allowed to cool to 50°C before proceeding. 2. Mix the contents of the test tubes and place them in a water bath kept at 40°C -50°C. 3. Keep the tubes in the water bath for 5 minutes, shaking once or twice to promote mixing. Air-cool for 5 minutes, then place in an ice bath. Observe for evidence of setting or solidifying. 4. Record your results in the table.
Transcribed Image Text:B. Action of Pancreatic Proteases and Trypsin on Protein 1. Prepare 3 test tubes containing 2 mL egg albumin solution. Tube 1: Add 1 mL 5% Trypsin Tube 2: Add 1 mL 5% Trypsin and 2 mL of 0.5% Na₂CO3 Solution Tube 3: Add 2 mL 0.5% Na₂CO3 Solution 2. Place all test tubes in hot water bath maintained at 38-40°C for one hour. 3. Filter the contents of each test tube and use the filtrate for biuret test. C. Action of Bromelain in the Setting of Gelatin 1. Label 5 test tubes from 1 to 5. Place the substances as indicated in the table below. Test Tube 1 (Control) 2 3 4 5 Gelatin 2 ml 2 ml 2 ml 2 ml Pineapple Juice 1 ml (boiled) 1 ml 1 ml Meat Tenderizer 0.25M MgCl2 5 drops 1 ml 1 ml Water 2 ml 5 mg a. A purified preparation of papain may be used instead of the meat tenderizer. b. Test tube 2 should be prepared by putting 1 ml of pineapple juice in the test tube and placing it in a boiling water bath for 5 minutes. The tubes should then be allowed to cool to 50°C before proceeding. 2. Mix the contents of the test tubes and place them in a water bath kept at 40°C -50°C. 3. Keep the tubes in the water bath for 5 minutes, shaking once or twice to promote mixing. Air-cool for 5 minutes, then place in an ice bath. Observe for evidence of setting or solidifying. 4. Record your results in the table.
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