FRAP (Fluorescence Recovery After Photobleaching) (A) 1 Immobile fraction Photoech 2 3 Recovery half time Time Bleach 6 Fluorescence intensity in bleach ROI Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently photobleached. The recovery of fluorescence was monitored and gave the following profile: 100 M S time a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your ranking based on their FRAP curves and the most probable appearance and composition of the three membranes. b. Draw the expected image of the surface of the three membranes before photobleaching and after fluorescence recovery as viewed under a fluorescence microscope. % fluorescence

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question
Answer questions a and b. DRAW THE BEFORE AND AFTER BASED ON QUESTION B.
FRAP (Fluorescence Recovery After Photobleaching)
(A) 1
Immobile fraction
Photoech
2
3
Recovery
half time
Time
Bleach
6
Fluorescence intensity in
bleach ROI
Transcribed Image Text:FRAP (Fluorescence Recovery After Photobleaching) (A) 1 Immobile fraction Photoech 2 3 Recovery half time Time Bleach 6 Fluorescence intensity in bleach ROI
Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently
photobleached. The recovery of fluorescence was monitored and gave the following profile:
100
M
S
time
a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of
your ranking based on their FRAP curves and the most probable appearance and composition
of the three membranes.
b. Draw the expected image of the surface of the three membranes before photobleaching
and after fluorescence recovery as viewed under a fluorescence microscope.
% fluorescence
Transcribed Image Text:Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently photobleached. The recovery of fluorescence was monitored and gave the following profile: 100 M S time a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your ranking based on their FRAP curves and the most probable appearance and composition of the three membranes. b. Draw the expected image of the surface of the three membranes before photobleaching and after fluorescence recovery as viewed under a fluorescence microscope. % fluorescence
Expert Solution
steps

Step by step

Solved in 3 steps with 2 images

Blurred answer
Knowledge Booster
Learning & Memory
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education