When you did Wright-Giemsa staining, you added the blue dye in the well containing C2C12 cells. The blue dye contains Select one: a. Methylene blue and 1X PBS b. Methylene blue, eosin and 10% formaldehyde C. Trypan blue d. Methylene blue and fluoromount What reagent do you use to sterilize your cell culture equipment and coverslips? Select one: a. hydrogen peroxide b. 1XPBS c. 70% ethanol d. 99% ethanol When you do not use your cells actively for research, you can freeze them to keep them safe and alive. What is the OPTIMAL temperature to store cells? Select one: a. -20°C freezer b. liquid nitrogen (below -130°C) c. -80°C d. -60°C

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Jenna Franchino
When you did Wright-Giemsa staining, you added the blue dye in the well
containing C2C12 cells. The blue dye contains_
Select one:
a.
Methylene blue and 1X PBS
O b. Methylene blue, eosin and 10% formaldehyde
C. Trypan blue
d. Methylene blue and fluoromount
What reagent do you use to sterilize your cell culture equipment and
coverslips?
Select one:
a. hydrogen peroxide
O b. 1XPBS
c. 70% ethanol
d. 99% ethanol
When you do not use your cells actively for research, you can freeze them to
keep them safe and alive. What is the OPTIMAL temperature to store cells?
at of
Select one:
O a.
-20°C freezer
b. liquid nitrogen (below -130°C)
C.
-80°C
O d
-60°C
MacBook Air
Transcribed Image Text:Request Request Jenna Franchino When you did Wright-Giemsa staining, you added the blue dye in the well containing C2C12 cells. The blue dye contains_ Select one: a. Methylene blue and 1X PBS O b. Methylene blue, eosin and 10% formaldehyde C. Trypan blue d. Methylene blue and fluoromount What reagent do you use to sterilize your cell culture equipment and coverslips? Select one: a. hydrogen peroxide O b. 1XPBS c. 70% ethanol d. 99% ethanol When you do not use your cells actively for research, you can freeze them to keep them safe and alive. What is the OPTIMAL temperature to store cells? at of Select one: O a. -20°C freezer b. liquid nitrogen (below -130°C) C. -80°C O d -60°C MacBook Air
Expert Solution
Step 1

Lyophilization is a process of freeze-drying the sample such as cells and tissues under a vacuum which makes the cells viable for mani ears. the water molecules are removed slowly not to damage the cell components and it is directly frozen to a solid phase. 

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