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- Demonstration of Osmosis using Potato Osmometer 1. What will happen when the liquid in the cavity overflows? ExplainDiscuss in chemical detail the mechanism of action (how it works) of IMAC. Compare and contrast gel filtration chromotography with IMAC. Discuss the pros and cons for each technique. Why would a scientist prefer one technique over another? In what situations would one technique be more viable than the other?Gel-filtration chromatography separates molecules according to their size . Smaller molecules diffuse faster in solution than larger ones, yet smaller molecules migrate more slowly through a gel- filtration column than larger ones. explain this paradox. What should happen at very rapid flow rates?
- EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Procedure for Cryopreserving MCF-7 and THP-1 Cells• Ensure that the culture is up to the late log phase (assumption is made based on the cell concentration). Prepare the cell suspension and determine the cell concentration.• Dilute the cell suspension in 1:1 ratio with freezing medium (final concentration approximately 1 X 106 – 1 X 107cells/ml is desired). • Dispense the cell suspension into prelabeled cryovial and place the vial in -80°C freezer overnight (temporary storage).• Transfer the cryovial into liquid nitrogen cryotank for long term storage the following day.• After day 3, determine the cell concentration in the freezing medium andcompare with that of the stock concentration. Question: Please briefly explain what is cryopreservation(1), how procedure above are useful to make this experiment successful(2)POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What can you conclude by comparing potato A and potato D? 2. The liquid in the cavity of some potatoes came from where? 3. What is your conclusion about the experiment?POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What is your observation from potato A to D? 2. What can you conclude by comparing potato A and potato B? 3. What can you conclude by comparing potato A and potato C?
- a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.Observation of Microscopic Cellular TransportWhy is Endo Agar is recommended for membrane filtration experiment and not TSA plate?
- Calculate the estimated concentration of test agent that inhibit cell growth by 50% ( GI50) valueStandard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2 mg/ml of lysozyme and you have 2 ml in the vial. You will want to have a minimum of 1 ml of each concentration for the spectrophotometer. How would you proceed? (Saline is added to make the various concentrations). Concentration Am't stock lysozyme or am't of a previous dilution (note which) Amount saline Total volume wanted 1.00 mg/ml 0.75 mg/ml 0.50 mg/ml 0.375 mg/mlActivity 3b - Diffusion across a semipermeable membrane 1. Define selective permeability. 2. Explain the properties of the cell membrane that make it selectively permeable. Activity 3c - Osmosis in potatoes 1. Define osmosis. 2. Define hypertonic, hypotonic, and isotonic. Hypertonic Hypotonic Isotonic Activity 3d - Osmosis in plant cells 1. Fill in the table below to indicate what happened to the elodea cells in each of the following solutions. Solution Hypertonic Isotonic Hypotonic Will the cell membrane swell or shrivel? What happens to the chloroplasts in the hypotonic solution? Why do you think that happens?