Experiment Table 1 Tube 8 7 6 5 4 3 2 1 Lab 01: Measurement and the Standard Curve 1 Standard Curve Final Protein Concentration 50 ug/ml. 25 µg/ml 15 ugiml 10 µg/ml 7.5 ugl 5.0 µg/ml 2.5 µg/mL 0 µg/ml PL of BSA protein stock (0.5 mg/mL) Am IMGal 10.05 Stil 10.02 30L 10.02ml 2044 10.415ml Bul 10.0lm 1.005mL Oml Bradford 0.15M NaCl for 1.0 mL final volume Reagent 900 pl 900 μL 900 L 900 μL 900 μL 900 μL sul 900 µl 900 μL OOSAL SOUL 10.07ML 1OYOL L 0.0xGmL SUL Obal Que Sul مان 100
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- The order is for 1000ml of 5% D/W, 1000ml of Ringers Lactate, 500ml of NS to run over a 36 hour period. The drop factor is 15gtt/ml. How many gtt/min will you administer?1&3/4 tbs po BID x 7 days #QS How many milliliters should the pharmacy dispense? this isnt 364 mL08._Order: 40,000 units heparin in 1L of 0.9% NaCl to infuse at 25 mL/h. Calculate the hourly heparin dosage in mL/hour.
- Order: 1L of D5W to infuse over 6 hrs Available: Macrodrip set: 10gtt/mL The flow rate would be Order: 1000mL of D51/2NS with 1 ample of multiple vitamins to infuse over 8 hrs Available: Macrodrip set: 15gtt/mL The flow rate would be NDC 25021-131-12 Order: metronidazole 500mg IV Q6hrs to infuse over 30 minutes Available: Metronidazole Injection, USP 500 mg per 100 ml. (5 mg per mL) Protect from light until use. For Intravenous Infusion only. 25021 13182 Lot: AGENT The flow rate would be 102mL Blony Circ And [x-e. Single doct Exp. gtt/ min. bi gtt/min. mL/hr.250- 1 2 3 4 5 6 -250 150- -150 100- -100 75- -75 50- 37- 25- 20- 15- 10 10- Std Biorad BSA Calf serum Fibrinogen Std Biorad Transferrin 161-0318 161-0374 -50 -37 -25 -20 15 10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data - measured distance moved and calculated Rr values Item Band Distance moved (mm) Rf Log 10 Mr (from graph) Calculated Mr (antilog of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1EcoRI 4359 Aatll - Zral 4284 Bei 4209 BsrBl 4205 Clal - BspDI 23 Hindill 29 EcoRV 185 Bmtl - Nhel 229 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Hincll 3905 Scal 3844 BamH 375 Sgrl 409 Banll 471 Banll 485 Вы 3787 Bsl 3759 Bgl 528 Sphl 562 EcoNI 622 Sal - Acct - Hincll 651 Pvul 3733 Pstl 3607 Bartl 3602 Pshl 712 Asel 3537 Eagl 909 Bell 949 Bsal 3433 BarDI 3420 Nrul 972 PBR322 4,361 bp BstAPI 1045 Ahdi 3361 BspMI - Bfual 1063 PAMI 1315 Bsml 1353 PAMI 1364 Acul 3000 Ori Styl 1369 Aval - BsoBl 1425 PpuMI 1438 Msel 1444 Bigl 1447 Ppu 1480 AlwNI 2884 Bell 2777 kI 2682 rop Drdi 2575 Bsgl 1650 BspEI 1664 Pcil - Afl 2473 rBl 2404 Earl 2351 Bspol - Sapl 2350 Ndel 2295 BstAPI 2291 Bsaß 1668 Xmat 2029 Pvull 2064 BsmBI 2122 Dndl 2162 BstZ171 - Acct 2244 Bsal 2225 TthI- PI 2217 Figure 1 If your gene of interest was inserted at the Sphl restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.