EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Procedure for Cryopreserving MCF-7 and THP-1 Cells • Ensure that the culture is up to the late log phase (assumption is made based on the cell concentration). Prepare the cell suspension and determine the cell concentration. • Dilute the cell suspension in 1:1 ratio with freezing medium (final concentration approximately 1 X 106 – 1 X 107cells/ml is desired). • Dispense the cell suspension into prelabeled cryovial and place the vial in -80°C freezer overnight (temporary storage). • Transfer the cryovial into liquid nitrogen cryotank for long term storage the following day. • After day 3, determine the cell concentration in the freezing medium and compare with that of the stock concentration. Question: Please briefly explain what is cryopreservation(1), how procedure above are useful to make this experiment successful(2)

Q: Which of the following contributes the least to the rapid spread of antibiotic resistance among…

A: Antibiotics are some kind of medicines that helps in preventing infections that can be developed by…

Q: transfer and isolation techniques: What is the reason for flaming the tubes before and after each…

A: Usage of aseptic techniques is very important when it comes to the transfer and isolation of…

Q: What does an ‘I’ result mean in disk diffusion susceptibility test?

A: Susceptibility tests determine a microbe's vulnerability to antimicrobial drugs by exposing a…

Q: Based on the following information acquired using a hemocytometer, calculate: i) cell concentration*…

A: In a hemocytometer, both the stained (viable) and unstained (non-viable) cells are counted in…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell fractionation is a process that involves rupturing cells, separating and suspending cellular…

Q: (b) Define concentration polarization and polarization modulus. How is polarization modulus…

A: CP is a process of accumulation of retained solutes in the membrane boundary at the feed side. It is…

Q: Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture…

A: Any form of white blood cell that is found in the spleen or has been separated from splenic tissue…

Q: Why is it so important to not vortex and/or pipette vigorously after you lyse the cells during the…

A: Mini prep is a protocol used in the isolation of bacterial plasmid DNA. It is a very rapid technique…

Q: Questions 1. The data from the experiment Dr. Trudeau describes is shown below in Figure 1. Graphs…

A: The experiment has done with three kinds of rabbits. The rabbits are placed in three different…

Q: inical application: A 44-year-old man with HIV is receiving antibiotics through a intravenous…

A: HIV or human immunodeficiency virus is a virus causes AIDS in humans. HIV contains single stranded…

Q: Gram staining principle a. Describe the structure of peptidoglycan b. Describe in detail the…

A: Answer: a)The crystal lattice of peptidoglycan is composed of two amino sugars that alternately bond…

Q: The figures below are the results of flow cytometer analysis of a drugs (camptotechin) effect on…

A: Flow cytometry is a technique that can be used to detect apoptotic activity in a cell.  Apoptosis is…

Q: Biochemical and differential tests can be incorporated into growth media or performed on isolated…

A: It is required to provide three examples of differential test that is incorporated into growth media…

Q: Questions 8 - What tonicity would best describe Beaker E and why? Questions 9 - What tonicity would…

A: Homeostasis means maintaining balance. Beaker A fill with 1000 ml water, that means beaker A doesn't…

Q: Compute for the bacteria/ml of raw milk at dilution factor of 100, if bacterial cell counts in 10…

A: Bacteria  Sub microscopic organisms that are found almost every where on earth. They may be useful…

Q: In the 1960s, bald eagles in the United States were at the brink of extinction. Scientists…

A: The answer for this question will be option B.

Q: Bio If you plated 100 uL of bacteria from a 10-5 dilution and count 73 colonies after incubating…

A: To calculate the CFU/mL (Colony-Forming Units per milliliter) of the original (undiluted) culture,…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Q: You have learned quite a number of different physicochemical methods for fractionating the contents…

A: Proteins are large, complex molecules that are fundamental to the structure and function of living…

Q: indicated by the arrow. A. What is the name of this part? B. What is the function performed by this…

A: The image given in the question is of a microscope which helps to view the specimen which is not…

Q: (ii) Complete the table comparing the light and electron microscopes. The table should fit on a…

A: Difference between magnification and resolution Resolution is the ability of optical equipment to…

Q: Distance of color diameter (mm) 20 15 KMNO, 0.1M - KMNO, 1.0M - 5. 15 45 09 75 Time (minutes) 06

A: * The molecules movement in  agarose gel is dependent on the  size  charge  *And pore size also…

Q: Which fractions measured from your gel filtration experiment had the most and least amount of…

A: Gel filtration chromatography or size exclusion chromatography is a technique used to separate…

Q: Ja • This is micrograph of a Gram stained bacterium. Look at the cells indicated by arrows, answer…

A: Bacteria are single-celled bacteria that exist in a variety of settings. They can take many forms,…

Q: Explain briefly why choosing a specific standard is less important for determining the concentration…

A: A standard is a commonly accepted and agreed-upon measurement or criterion used for comparison or…