EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Procedure for Cryopreserving MCF-7 and THP-1 Cells • Ensure that the culture is up to the late log phase (assumption is made based on the cell concentration). Prepare the cell suspension and determine the cell concentration. • Dilute the cell suspension in 1:1 ratio with freezing medium (final concentration approximately 1 X 106 – 1 X 107cells/ml is desired). • Dispense the cell suspension into prelabeled cryovial and place the vial in -80°C freezer overnight (temporary storage). • Transfer the cryovial into liquid nitrogen cryotank for long term storage the following day. • After day 3, determine the cell concentration in the freezing medium and compare with that of the stock concentration. Question: Please briefly explain what is cryopreservation(1), how procedure above are useful to make this experiment successful(2)

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