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- discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.What is the principle of freezing of cell lines and primary cells.Describe the general protocol for freezing cultured cells.In detail.What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?
- What is the principle of freezing of cell lines and primary cells. Describe the general protocol for freezing cultured cells.Explain the statements below: 1.Animal cell cultures requires high technical skills to interpret and to regulate animal cell culturing. 2. Animal cell culture makes use of low amounts of reagents.Guide Questions: Differentiate the types of culture media based on its physical state and uses. What makes agar good support for microbial growth?
- Define the following termonologies in brief : Focal Adhesion Biofilm Calcification and examples in implanted biomaterials Non-fouling surface AptamerDefine the following terms; Sterilization, and Aseptic Technique. What are the differences between the two concepts?Outline a method for preparation of tomato cell suspension culture and subculture
- 1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions. 1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixtureIn a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with mold