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transfer and isolation techniques:
What is the reason for flaming the tubes before and after each transfer?
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- Briefly describe the rationale and method of color compensation in flow cytometry.Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…Nursing question [3. make table to differentiate ITP, TTP, HIT, DIC.
- INTERPRETATION OF RESULIS NEGATIVE: Two lines appear. One colored line should be in the control region (C), and another apparent colored or faded color line adjacent should be in the test region (T). This negative result indicates that the drug concentration is below the detectable level. POSITIVE: One colored line appears in the control region (C). No line appears in the test region (T). This positive result indicates that the drug concentration is above the detectable level. INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test panel. If the problem persists, discontinue using the lot immediately and contact your local distrībutor. READING OF RESULTS INTERPRETATION SONTROL TEST SAMPLEAPPLIED PROJECT CONTROLLING RED BLOOD CELL LOSS DURING SURGERY A typical volume of blood in the human body is about 5 L. A certain percentage of that volume (called the hematocrit) consists of red blood cells (RBCS); typically the hematocrit is about 45% in males. Suppose that a surgery takes four hours and a male patient bleeds 2.5 L of blood. During surgery the patient's blood volume is maintained at 5 L by injection of saline solu- tion, which mixes quickly with the blood but dilutes it so that the hematocrit decreases as time passes. 1. Assuming that the rate of RBC loss is proportional to the volume of RBCS, determine the patient's volume of RBCS by the end of the operation. 2. A procedure called acute normovolemic hemodilution (ANH) has been developed to minimize RBC loss during surgery. In this procedure blood is extracted from the patient before the operation and replaced with saline solution. This dilutes the patient's blood, resulting in fewer RBCS being lost during the…K.What would happen if you reversed the electrodes on the Gel box? What would you get look like after imaging?
- What kind of cells are suitable for flow cytometric analysis and why? Please answer with 5 dot points/sentences minimuma 55 -year-old man who has upon catheterization two-vessel CAD. He has adverse effects on nitrates and refused to use them again because they cause severe headaches. His medical history includes asthma and hyperlipidemia. What drug do you choose for him? Specify the name of the drug or its specific class? And Why?Question: The physician orders 1 liter of D5LR to infuse at 83 mL/hr. The IV tubing delivers 15 gtt/mL. Calculate the drip rate.
- There are three main subsystems to a flow cytometer. Describe the primary function of each one in a short phrase or sentence. Please answer with 9 dot points/sentences minimumPrecipitin test is typically performed to identify the origin of blood. What are the advantages of using the gel diffusion method over the others (capillary method and electrophoretic method)?Briefly explain the technique for preparation and interpretation of disc diffusion tests.