Indicate 3 ways of visualizing a TLC after elution. Name three types of chromatography (excluding TLC
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Indicate 3 ways of visualizing a TLC after elution.
Name three types of chromatography (excluding TLC)
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- Identify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or class of lipids that are detected using these methods.Describe agglutination reaction test used to determine A, B , AB or O blood types. Must explain each agglutination reactions involving A, B, AB and O blood type and all antigens and antibodies involved in each reaction.Choose correct option and explain shortly. Question:- Which of the following is the best description of how you can measure white blood cells (WBCs) using the fluorescent light application of a flow cytometer? (a) How granular each cell is and what surface proteins they express, but not how big they are (b) How granular each cell is, but not how big they are or what surface proteins they express (c) How big each cell is, but not how granular they are or what surface proteins they express (d) How big and how granular each cell is, but not what surface proteins it expresses (e) How big each cell is and what surface proteins they express, but not how granular they are (f) What surface proteins each cell expresses, but not how big or how granular they are
- The growth of hybridoma cells, producing a monoclonal antibody, was monitored for 4 and a half days, and the results obtained are listed in the following table: time (days) nº cel/mL x 106 ln nº cel 0 0,45 -0,79851 0,2 0,52 -0,65393 0,5 0,65 -0,43078 1 0,81 -0,21072 1,5 1,22 0,198851 2 1,77 0,57098 2,5 2,13 0,756122 3 3,55 1,266948 3,5 4,02 1,391282 4 3,77 1,327075 4,5 2,2 0,788457 What is the maximum specific rate (µmax) of growth of these cells per day? what is the doubling time of these cells (in days)?What is the indication of violet blue color in biuret test? Explain in 1-3 sentencesGive the color reaction in Wright's stain and the function of these cells. CELL COLOR REACTION IN FUNCTION WRIGHT'S STAIN RED BLOOD CELL NEUTROPHIL NUCLEUS: CYTOPLASM/GRANULES: EOSINOPHIL NUCLEUS: CYTOPLASM/GRANULES: BASOPHIL NUCLEUS: CYTOPLASM/GRANULES: LYMPHOCYTE NUCLEUS: CYTOPLASM/GRANULES: MONOCYTE NUCLEUS: CYTOPLASM/GRANULES: PLATELET CYTOPLASM/GRANULES:
- Yasuo Yamagata, a 63-year-old, experienced severe back pain for several weeks before visiting his family physician. He also complained of fatigue and looked pale. Blood analysis revealed a red blood cell count of 3.2 × 106/μl (normal 4.2–5.0 × 106/μl), a white blood cell count of 2800/μl (normal 5000/μl), a sedimentation rate of 30 mm/h (normal <20 mm/h) and a serum IgG of 4500 mg/dl (normal 600–1500 mg/dl). IgA and IgM levels were well below normal. Skeletal survey showed lytic lesions in vertebrae, ribs and skull. A bone marrow sample revealed 75% infiltration with plasma cells. Elevated protein in urine was confirmed to be Bence-Jones protein (immunoglobulin light chains). The patient was diagnosed with IgG λ multiple myeloma and began an immediate chemotherapy regime. Which of the following would be consistent with this type of malignant tumor of plasma cells? a. Serum IgG is polyclonal. b. Anemia and neutropenia are present as the result of plasma-cell infiltration in the…Phenotype: A2B 1. Give the possible genotype/s of A:B 2. If you were to perform the blood typing of this individual, what are the expected results given the following reagents Phenotype Forward typing (reagents) Reverse typing (reagents) Anti-A Anti-B Anti-H A cells B cells Al Write "+" for presence of agglutination and "-" for absence of agglutination 3. Explain the results of the blood typing, why would there be a "+" or a "" reaction during the testing. Relate your answer to the presence/absence of antigens and/or antibodies2:11 Lab 6_BLOOD_assignment_Uni... •.. 2 of 2 Lab 6: BLOOD (supplement page) Identify 1 example of each formed element (including the platelet) in this figure. https://www.istockphoto.com/photo/human-blood-cell-under-microscope-1000x-gm472212721-36663142
- Describe the changes in clotting and bleeding balance during disseminated intravascular coagulation (DIC). Describe the most important blood coagulation tests used to monitor the patients receiving heparin and warfarin. Distinguish between immediate and delayed hypersensitivity. What drugs are used to treat immediate hypersensitivity and how do these drug work? Why don’t these compounds work in treating delayed hypersensitivity?A. Differentiate the chemistry and function of primary and secondary antibodies in ELISA. B. Discuss the purpose of adding a blocking buffer. What happens when you fail to add the blocking buffer? Why do we use BSA as the primary component of the blocking buffer?Provide the correct susceptibility rating
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