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1. What is the independent variable in the experiment? Why? 2. What is the dependent variable in the experiment? Why? 3. What possible variables should you be sure to hold Constant? Give three and explain why. 4. What is your control variable? Why is this a valid control?

1. What is the independent variable in the experiment? Why? 2. What is the dependent variable in the experiment? Why? 3. What possible variables should you be sure to hold Constant? Give three and explain why. 4. What is your control variable? Why is this a valid control?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Direction: Read and analyze the following laboratory experiment and answer the following question. PART 3: PLASMOLYSIS Materials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps, compound microscope, iodine, small knife, water, salt (5% and 10% solution) Methodology: 1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife. 2. Use an eye dropper to place a drop of water in the center of a microscope slide. Use the tweezers to peel a thin layer of skin tissue from the thick part of the onion wedge and place it in the center of the microscope slide. 3. 4. 5. Add a drop of water and a drop of iodine over the onion tissue on the slide. Carefully lower a cover glass slip at an angle on the stained tissue to allow air bubbles to escape. 6. Examine the prepared slide under the compound microscope at 100X magnification. 7. Record what the cells look like. 8. Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per 100 ml of distilled water in a beaker. Stir until dissolved. Also prepare a 10% solution by adding 10 grams of salt per 100 ml of distilled water in another beaker. 9. Use a dropper to add a few drops of the 5% solution to one side of the cover slip of your prepared slide. The 5% solution should mix with the fluid already on your onion tissue slide. 10. Carefully slide the triangle shaped end of a paper towel under the opposite end of the cover slip. This will cause the liquid to mix more with the fluid on the slide. 11. Use additional drops of 5% solution as needed to complete the introduction of the new solution. 12. Repeat steps 7 and 8. 13. Use a dropper to add a few drops of the 10% solution to one side of the cover slip of your slide with the 5% solution. The 10% solution should mix with the fluid already on your onion tissue slide. 14. Carefully slide the triangle shaped end of a paper towel under the opposite end of the cover slip. Use additional drops of 10% solution as needed to complete the introduction of the new solution. 15. Repeat steps 7 and 8. Questions: 1. What is the independent variable in the experiment? Why? 2. What is the dependent variable in the experiment? Why? 3. What possible variables should you be sure to hold Constant? Give three and explain why. 4. What is your control variable? Why is this a valid control?
Transcribed Image Text:Direction: Read and analyze the following laboratory experiment and answer the following question. PART 3: PLASMOLYSIS Materials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps, compound microscope, iodine, small knife, water, salt (5% and 10% solution) Methodology: 1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife. 2. Use an eye dropper to place a drop of water in the center of a microscope slide. Use the tweezers to peel a thin layer of skin tissue from the thick part of the onion wedge and place it in the center of the microscope slide. 3. 4. 5. Add a drop of water and a drop of iodine over the onion tissue on the slide. Carefully lower a cover glass slip at an angle on the stained tissue to allow air bubbles to escape. 6. Examine the prepared slide under the compound microscope at 100X magnification. 7. Record what the cells look like. 8. Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per 100 ml of distilled water in a beaker. Stir until dissolved. Also prepare a 10% solution by adding 10 grams of salt per 100 ml of distilled water in another beaker. 9. Use a dropper to add a few drops of the 5% solution to one side of the cover slip of your prepared slide. The 5% solution should mix with the fluid already on your onion tissue slide. 10. Carefully slide the triangle shaped end of a paper towel under the opposite end of the cover slip. This will cause the liquid to mix more with the fluid on the slide. 11. Use additional drops of 5% solution as needed to complete the introduction of the new solution. 12. Repeat steps 7 and 8. 13. Use a dropper to add a few drops of the 10% solution to one side of the cover slip of your slide with the 5% solution. The 10% solution should mix with the fluid already on your onion tissue slide. 14. Carefully slide the triangle shaped end of a paper towel under the opposite end of the cover slip. Use additional drops of 10% solution as needed to complete the introduction of the new solution. 15. Repeat steps 7 and 8. Questions: 1. What is the independent variable in the experiment? Why? 2. What is the dependent variable in the experiment? Why? 3. What possible variables should you be sure to hold Constant? Give three and explain why. 4. What is your control variable? Why is this a valid control?
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