How does temperature affect how lactase drops work to break down lactose into glucose and galactose?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Describe what you would do in your experiment. For each step, explain why the step is
important. Add more steps if required.
STEP
STEP:
WHY THIS STEP IS IMPORTANT:
Measure the milk
To ensure that the amount of milk used in
the hot and cold bath are the same give an
accurate result.
Place the milk in a test tube and put it in the
To observe the effect of temperature
change and how it affects the lactase.
hot bath, in the cold bath and one with no
temperature change.
Place the thermometer to the test tube add
This is to check if you have the adequate
drops of lactase into the three test tubes
temperature that you're looking for.
Add drops of lactase into the three test tubes Doing this after the adding them into the
different baths would give an accurate
result
Using the glucose test strips to see the effect
This is important to see how well the
lactase worked in each environment and
record it.
If you actually conducted this experiment, what safety issues would you need to identify?
If doing this experiment you would wear safety goggles to protect your eyes, gloves, a lab coat and tie your hair
if you have long hair. This is to protect you from spills and since the lab involves hot water you would need to be
next to a sink incase of a hot water spill.
If you actually conducted this experiment, what appropriate precautions would you take to ensure that you
performed the experiment safely?
You would wear gloves and goggles against spill, measure liquids at eye level for an accurate result
If you actually conducted this experiment, what results would you expect to see?
If this experiment was conducted you would expect to see the milk in the hot bath not react. And the one in the
cold bath would also react slower
Why would you expect these results?
These results would be possible because at a higher than optimal temperature the enzyme would get denatured
causing it to change shape and prevent substates from binding to the active site. And the one in the cold bath
would be slow because below optimal temperature the molecules would move slower causing the reaction to
get slower.
Transcribed Image Text:Describe what you would do in your experiment. For each step, explain why the step is important. Add more steps if required. STEP STEP: WHY THIS STEP IS IMPORTANT: Measure the milk To ensure that the amount of milk used in the hot and cold bath are the same give an accurate result. Place the milk in a test tube and put it in the To observe the effect of temperature change and how it affects the lactase. hot bath, in the cold bath and one with no temperature change. Place the thermometer to the test tube add This is to check if you have the adequate drops of lactase into the three test tubes temperature that you're looking for. Add drops of lactase into the three test tubes Doing this after the adding them into the different baths would give an accurate result Using the glucose test strips to see the effect This is important to see how well the lactase worked in each environment and record it. If you actually conducted this experiment, what safety issues would you need to identify? If doing this experiment you would wear safety goggles to protect your eyes, gloves, a lab coat and tie your hair if you have long hair. This is to protect you from spills and since the lab involves hot water you would need to be next to a sink incase of a hot water spill. If you actually conducted this experiment, what appropriate precautions would you take to ensure that you performed the experiment safely? You would wear gloves and goggles against spill, measure liquids at eye level for an accurate result If you actually conducted this experiment, what results would you expect to see? If this experiment was conducted you would expect to see the milk in the hot bath not react. And the one in the cold bath would also react slower Why would you expect these results? These results would be possible because at a higher than optimal temperature the enzyme would get denatured causing it to change shape and prevent substates from binding to the active site. And the one in the cold bath would be slow because below optimal temperature the molecules would move slower causing the reaction to get slower.
Which question will your experiment investigate? (select one and delete the others)
How does temperature affect how lactase drops work to break down lactose into glucose and
galactose?
What variable will you change in this experiment? This is also called the Independent variable.
How will you change it?
What variable will you measure in this experiment to determine the effect of the variable you
changed? This is also called the Dependent variable.
How will you measure it?
The temperature is the variable that would be changed and it would be changed by placing
the milk in a hot bath and another one in an ice bath
What variables will you keep the same in this experiment?
milk (lactase)
Check off the items in this list that you would use for this experiment. You may add any items
you feel you would need.
O skim milk (which contains lactose)
O Lactase drops
O Graduated cylinder
O Eye dropper
O Ice cubes
O pH paper
O Hot plate
O Mixing spoon
O Thermometer
O Vinegar (acidic)
O Test tubes
O Glucose test strips
O Baking soda (basic)
O Other items you feel you would need to use for this experiment.
Transcribed Image Text:Which question will your experiment investigate? (select one and delete the others) How does temperature affect how lactase drops work to break down lactose into glucose and galactose? What variable will you change in this experiment? This is also called the Independent variable. How will you change it? What variable will you measure in this experiment to determine the effect of the variable you changed? This is also called the Dependent variable. How will you measure it? The temperature is the variable that would be changed and it would be changed by placing the milk in a hot bath and another one in an ice bath What variables will you keep the same in this experiment? milk (lactase) Check off the items in this list that you would use for this experiment. You may add any items you feel you would need. O skim milk (which contains lactose) O Lactase drops O Graduated cylinder O Eye dropper O Ice cubes O pH paper O Hot plate O Mixing spoon O Thermometer O Vinegar (acidic) O Test tubes O Glucose test strips O Baking soda (basic) O Other items you feel you would need to use for this experiment.
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