B. Color Reactions Test Biuret test Samples Added reagent/s Observation and Interpretation
B. Color Reactions Test Biuret test Samples Added reagent/s Observation and Interpretation
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![B. Color Reactions
Test
Biuret test
Hopkins-Cole test
Millon's test
Reduces Sulfur test
Samples
Added reagent/s
Observation and
Interpretation](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F379e497c-5023-43cd-aa11-13c247df7985%2Fc6663efa-0df2-4e1c-baf2-083c71576d8d%2Fm0vbxi_processed.jpeg&w=3840&q=75)
Transcribed Image Text:B. Color Reactions
Test
Biuret test
Hopkins-Cole test
Millon's test
Reduces Sulfur test
Samples
Added reagent/s
Observation and
Interpretation
![B. Color Reactions (Use 2mL of the Protein solution)
1. Biuret test - Mix 2mL of egg albumin solution with 1mL of dilute NaOH and 10 drops of diluted
CuSO4 solution in a test tube. Shake well. Note the change in color.
2. Millon's test - To 2mL egg albumin, add 10 drops of Millon's reagent. Shake well and heat over a
water bath to boiling. Note the color change.
3. Hopkins-Cole test - To 5 drops of egg albumin solution, add 5 drops of glyoxylic acid. Carefully run
down 5 drops of conc. H₂SO4 on the side of the test tube. Note the color formed at the point of
contact between the two liquids.
4. Reduces Sulfur test - To 2mL of egg albumin solution in a test tube, add 1mL of 20% NaOH and
10 drops of 10% Lead acetate solution. Cover the test tube with a cork and boil in a water bath for
two minutes. Observe the color change and explain.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F379e497c-5023-43cd-aa11-13c247df7985%2Fc6663efa-0df2-4e1c-baf2-083c71576d8d%2F72n9ahh_processed.jpeg&w=3840&q=75)
Transcribed Image Text:B. Color Reactions (Use 2mL of the Protein solution)
1. Biuret test - Mix 2mL of egg albumin solution with 1mL of dilute NaOH and 10 drops of diluted
CuSO4 solution in a test tube. Shake well. Note the change in color.
2. Millon's test - To 2mL egg albumin, add 10 drops of Millon's reagent. Shake well and heat over a
water bath to boiling. Note the color change.
3. Hopkins-Cole test - To 5 drops of egg albumin solution, add 5 drops of glyoxylic acid. Carefully run
down 5 drops of conc. H₂SO4 on the side of the test tube. Note the color formed at the point of
contact between the two liquids.
4. Reduces Sulfur test - To 2mL of egg albumin solution in a test tube, add 1mL of 20% NaOH and
10 drops of 10% Lead acetate solution. Cover the test tube with a cork and boil in a water bath for
two minutes. Observe the color change and explain.
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