. Amino acids, which are constituents of proteins, are often separated by paper chromatography. Twoamino acids, valine and lysine, are shown below as they would appear inside the body. Predict whichone would have the highest R, value by paper chromatography and explain why.COO¯+H3N- C-HCH-CH31CH3ValineCOO-+H3N- C-HCH2-CH2-CH2-CH2-NH3+Lysine

Predicting the Rf ValuesIn a typical chromatography setup with a non-polar solvent, non-polar…

Answered: . Amino acids, which are constituents…

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter21: Lipids

Section: Chapter Questions

Problem 21.5P

See similar textbooks

Similar questions

1
Question 32 Use the following chromatogram of standard amino acids: 24 11 16 19 3 12 21 15 6, 8 9 19 23 2 13 14 17 18 24 22 10 12 14 16 18 20 22 24 26 Time (min) Which of the following statements BEST describe the given chromatogram? O Peaks 4, 5, 11 and 24 have the highest concentrations in the mixture and highest affinity to the mobile phase. Peaks 14, 18, 20 and 22 have the lowest concentrations and highest affinity to the mobile phase. O The peaks are narrow and show clear separation of the mixture. The peaks are narrow and show suboptimal conditions for separation. (Au)
Can someone check my answers and see if they are correct?
A mixture of seven amino acids (glycine, glutamate, leucine, lysine, alanine, isoleucine, and aspartate) is separated by chromatography. Explain why only six spots show up when the chromatographic plate is coated with ninhydrin and heated.
Calculate the number of millimoles(mmol)in 500 mg of each of the following amino acids: alanine (MW=89), leucine (131), tryptophan (204), cysteine (121), and glutamic acid (147).
ef Correlate the structures of the standard food dyes to their relative position in the paper chromatogram. Refer to the structures shown below. H3C. *Na O O HO. O CH3 Allura Red AC (pigment in red dye) 01510 106 FO O Nat O Nat O Nat FO O Na+ 0=8=0 HO 0=S=0 Na Na+ Tartrazine (pigment in yellow dye) Brilliant Blue FCF (pigment in blue dye) What are the identities of the unknowns in paper chromatography of food dyes? How did you arrive at this conclusion? Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the experiment you did in thin layer chromatography of plant pigments? How do you say so?
A student performed a chromatography experiment to determine the Rf value of an unknown amino acid. The distances traveled by the amino acid and the solvent line were 3.0 cm and 8.5 cm, respectively. What is the Rf value for this amino acid? 0.3 cm O 0.35 1.8 5.5 0.85 cm 0.65 O 2.8
What is the best Chromatography instrument for analysis of Soluble Protein molecules CG-FID GC-MS LC-MS IC-MS O IC-EC
The amino acid Asp is triprotic with pK₁ = 1.88, pK₂ = 9.60, and pK3 = 3.65. What is the charge of the a-NH3* functional group at pH 11? -1 What is the net charge of Asp at pH 11? [Select] = The amino acid Asp is triprotic with pK1: 1.88, pK2 = 9.60, and pK3 = 3.65.
Propose a step-by-step procedure to separate the amino acids glycine and tyrosine mixture using Thin Layer Chromatography. Include materials and chemicals required.
The image below is a positive ionization mode Electrospray ionization-mass spectrum (ESI-MS) spectrum for a peptide, obtained using high resolution mass spectrometry. If ionization occurs through de-protonation (assuming that the mass of a proton is 1.0 Da), what is the neutral mass of the peptide, in Da? 627.271 627.604 100 - 627.938 628.271 628.604 628.938 627.0 628.0 629.0 mass/charge (m/z) 628.271 626.271 1878.813 O 1884.813 % Abundance
Given the Ksp = 1.4 x 10-13, what is the solubility of CoCO3 (i.e., what is the [Co2+] concentration) in water?

SEE MORE QUESTIONS

Recommended textbooks for you

Introduction to General, Organic and Biochemistry

Chemistry

ISBN:

9781285869759

Author:

Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:

Cengage Learning

Introduction to General, Organic and Biochemistry

Chemistry

ISBN:

9781285869759

Author:

Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:

Cengage Learning

SEE MORE TEXTBOOKS