Heparin is used as an anti-coagulant. A risk of heparin use is thrombocytopenia, or low platelet count. This risk is minimized with the use of low molecular weight heparins (LMWH), therefore it is desirable to separate LMWH from higher molecular weight heparins. The method of choice to do this is molecular exclusion chromatography. Below is a chromatogram from a molecular exclusion chromatographic run. Peaks ranging from A to J are clearly distinguishable. The heparin mixture that was analyzed had anywhere from 6 to 30 repeat units of monomer (where the heparin with 30 repeat units would be roughly five times the size of the heparin with six repeat units). a. Which letter most likely represents the peak with 6 repeat units given these heparin polymers were separated with molecular exclusion chromatography? b. Explain your reasoning describing the mechanism of retention in molecular exclusion chromatography. 100 80 60 60 Relative Abundance 40 40 E GH 20 20 B A 36 38 40 42 44 46 48 50 50 52 2 54 56 58 60 axis label on bottom is retention time (minutes) 60
Heparin is used as an anti-coagulant. A risk of heparin use is thrombocytopenia, or low platelet count. This risk is minimized with the use of low molecular weight heparins (LMWH), therefore it is desirable to separate LMWH from higher molecular weight heparins. The method of choice to do this is molecular exclusion chromatography. Below is a chromatogram from a molecular exclusion chromatographic run. Peaks ranging from A to J are clearly distinguishable. The heparin mixture that was analyzed had anywhere from 6 to 30 repeat units of monomer (where the heparin with 30 repeat units would be roughly five times the size of the heparin with six repeat units). a. Which letter most likely represents the peak with 6 repeat units given these heparin polymers were separated with molecular exclusion chromatography? b. Explain your reasoning describing the mechanism of retention in molecular exclusion chromatography. 100 80 60 60 Relative Abundance 40 40 E GH 20 20 B A 36 38 40 42 44 46 48 50 50 52 2 54 56 58 60 axis label on bottom is retention time (minutes) 60
Chapter4: Least-squares And Calibration Methods
Section: Chapter Questions
Problem 3P
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Transcribed Image Text:Heparin is used as an anti-coagulant. A risk of heparin use is thrombocytopenia, or low platelet
count. This risk is minimized with the use of low molecular weight heparins (LMWH), therefore it is
desirable to separate LMWH from higher molecular weight heparins. The method of choice to do
this is molecular exclusion chromatography. Below is a chromatogram from a molecular exclusion
chromatographic run. Peaks ranging from A to J are clearly distinguishable. The heparin mixture
that was analyzed had anywhere from 6 to 30 repeat units of monomer (where the heparin with 30
repeat units would be roughly five times the size of the heparin with six repeat units).
a. Which letter most likely represents the peak with 6 repeat units given these heparin polymers
were separated with molecular exclusion chromatography?
b. Explain your reasoning describing the mechanism of retention in molecular exclusion
chromatography.
100
80
60
60
Relative Abundance
40
40
E
GH
20
20
B
A
36 38 40 42 44 46 48 50
50
52
2
54 56 58 60
axis label on bottom is retention time (minutes)
60
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