Lab Report 4

During an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 140.1 mg of pure paracetamol was added to a 200 mL volumetric flask.   0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water 10 mL of the above solution was diluted to 100 mL   1)What is the concentration of the final diluted solution in mcg/mL?(1 decimal) 2) What volume of the diluted solution is required to prepare 100 mL of a 8 mcg/mL standard solution? (1 decimal)

Calculate the volume of NaOH used in each trial. Use sig figs.

analyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU) caffeine 1 1 4.67 302.85 aspartame 5 1 7.53 15.83 benzoic acid 1 1 8.14 89.98 saccharin 1 1 1.91 84.86 mixture(add everything above with 1:1:1:1 ratio)   1 4.47 69.58 How to get the concentration of the mixture in this case?

PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.

During an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 135.4 mg of pure paracetamol was added to a 200 mL volumetric flask. 0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water • 10 mL of the above solution was diluted to 100 mL a) What is the concentration of the final diluted solution in mcg/mL? Answer = mcg/mL (1 decimal) tem b) What volume of the diluted solution is required to prepare 100 mL of a 5 mcg/mL standard solution? Answer = mL (1 decimal) A Que

Please answer. Urgent.  Thank you.

▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? ▪ A) methanol B) Ethanol ▪ C) IPA

Data for finding log P of p-toluoyl penicillin Concentration of solution 1 is 0.001 M. The consequent solutions were prepared by two-fold serial dilution. Solution number Solution 1 Solution 2 Solution 3 Solution 4 Solution 5 Solution 6 Solution 7 Solution 8 Solution 9 Solution 10 Absorbance (342 nm) of octanol solution, initial 4 (overloaded) 4 (overloaded) 3.36 (too high) 1.658 0.831 0.482 0.268 0.173 0.086 (too low) 0.042 (too low) Absorbance(342 nm) of octanol solution, after shaking with water 1.650 0.810 0.452 0.253 0.154 Determination of logP from your data: Plot a calibration graph using initial absorbance data. Use this calibration graph to find concentrations of penicillin in octanol solutions after shaking them with water. The difference between initial and new penicillin concentrations in octanol solutions will give you amount of penicillin that moved into the aqueous phase in each case. Finally, calculate the log P for each solution using the following formula: logP = log-…

anch of the following components cannot be detected by gas chromatography? 4 Essential oils Alcohols b) Volatile compounds d) Non-volatile compounds 13- is measured as time elapsed from the point of injection to the peak maximum? a)-Column efficiency d)- Delectability c)-Retention time 14- Which of the following can be used to detect the components in gas chromatography a) non-volatile compounds b) volatile compounds c) Salts d) b&c 15-Which of the following methods is often referred to as mechanical separation processes. a)-Gas Chromatography b)-column chromatography c)- Sedimentation 16- For obtaining better resolution in TLC. and then developed with a second solvent at right angles to the first a) Stepwise b) Descending e) Ascending 17- This is defined as transport of electrically charged particles in a direct-current electric field a) ion exchange chromatography b) Electrophoresis c) ultracentrifuge d) exclusion 18-This figure indicates to schematic Diagram of a) Gas chromatography…

The amount of caffeine in an analgesic tablet was determined by HPLC using a normal calibration curve. Standard solutions of caffeine were prepared and analyzed using a 10-µL fixed-volume injection loop. Results for the standards are summarized in the following table. Concentration of Standards Signal (arbitrary units) (ppm) 50.0 8354 100.0 16925 150.0 25218 200.0 33584 250.0 42002 The sample was prepared by placing a single analgesic tablet in a small beaker and adding 10 mL of methanol. After allowing the sample to dissolve, the contents of the beaker, including the insoluble binder, were quantitatively transferred to a 25-mL volumetric flask and diluted to volume with methanol. The sample was then filtered, and a 1.00-mL aliquot was transferred to a 10-mL volumetric flask and diluted to volume with methanol. When analyzed by HPLC, the signal for the caffeine was found to be 21469. Report the number of milligrams of caffeine in the analgesic tablet.

Calculate the concentration of unknown red dye solution solution is not =12.9,134,12.55,0.34

3. Which of the following statements best describes a non-specific assay? directly measures the concentration of an analyte indirectly measures the concentration of an analyte O changes color as a function of analyte concentration measures the concentration of analyte using a absorption spectrometer

Compare and contrast the amount of caffeine you obtained from the single vs multipleextractions with Red Bull and Coca-Cola beverages. Explain why this has occured. Single Extractions:       TEST SOLUTION NAME      ABSORBANCE READING          [CAFFEINE]mg/100ml 5ml Red Bull                         2.412                  57.9mg/100ml 5ml Coca-Cola                         0.716                  16.2mg/100ml   Multiple Extractions:      TEST SOLUTION NAME        ABSORBANCE READING           [CAFFEINE]mg/100ml 10ml Red Bull                    3.253                      78.453 10ml Coca-Cola                     0.785                      17.844

A 10-mL aqueous solution of acetaminophen was extracted with 10 mL of octanol. The initial aqueous solution had an absorbance of 0.971 after 600 µL was diluted with 1400 µL of water. After extraction, undiluted aqueous phase had an absorbance of 1.047. Find Kow of acetaminophen. Kow = 0.01095

A 50.0 mL juice extract is colorimetrically assayed using Nelson's test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: 1mg/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926. A. What is the principle behind the assay? B. Calculate the concentration of the reducing sugar in the 50.0 mL sample in mg/mL. Apply Beer-Lambert's law: A=ɛbc. Computations: Answer:

please help with trial 1 so i can answer trial 2 and 3. thank you please show solution

. Given the following drugs in a urine specimen: Cocaine alkaloid Secobarbital (a derivative of barbituric acid) Carisoprodol (a neutral drug)      Devise an analytical flow chart that will be used to separate these substances from a urine specimen. The separation will be liquid extraction methods. Choose appropriate solvents and reagents based on the properties of the materials to be separated.

Plot a professional quality standard curve (absorbance vs. protein amount in µg) with an appropriate title and caption underneath the plot from the data give above. The caption should include the best-fit data.

Acetylcholine esterase, 3.0 mg, was dissolved in 10.0 mL solution. From this solution, 0.60 mL was added to the sample tube, brining the volume to a total of 3.0 mL. How many mg of acetylcholine esterase did you have in the sample tube?