The amount of caffeine in an analgesic tablet was determined by HPLC using a normal calibration curve. Standard solutions of caffeine were prepared and analyzed using a 10-uL fixed-volume injection loop. Results for the standards are summarized in the following table. Concentration of Standards Signal (arbitrary units) (ppm) 50.0 8354 100.0 16925 150.0 25218 200.0 33584 250.0 42002 The sample was prepared by placing a single analgesic tablet in a small beaker and adding 10 mL of methanol. After allowing the sample to dissolve, the contents of the beaker, including the insoluble binder, were quantitatively transferred to a 25-mL volumetric flask and diluted to volume with methanol. The sample was then filtered, and a 1.00-mL aliquot was transferred to a 10-mL volumetric flask and diluted to volume with methanol. When analyzed by HPLC, the signal for the caffeine was found to be 21469. Report the number of milligrams of caffeine in the analgesic tablet.

The amount of caffeine in an analgesic tablet was determined by HPLC using a normal calibration curve. Standard solutions of caffeine were prepared and analyzed using a 10-uL fixed-volume injection loop. Results for the standards are summarized in the following table. Concentration of Standards Signal (arbitrary units) (ppm) 50.0 8354 100.0 16925 150.0 25218 200.0 33584 250.0 42002 The sample was prepared by placing a single analgesic tablet in a small beaker and adding 10 mL of methanol. After allowing the sample to dissolve, the contents of the beaker, including the insoluble binder, were quantitatively transferred to a 25-mL volumetric flask and diluted to volume with methanol. The sample was then filtered, and a 1.00-mL aliquot was transferred to a 10-mL volumetric flask and diluted to volume with methanol. When analyzed by HPLC, the signal for the caffeine was found to be 21469. Report the number of milligrams of caffeine in the analgesic tablet.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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Question

The amount of caffeine in an analgesic tablet was determined by HPLC using a
normal calibration curve. Standard solutions of caffeine were prepared and analyzed
using a 10-µL fixed-volume injection loop. Results for the standards are summarized
in the following table.
Concentration of Standards
Signal
(arbitrary units)
(ppm)
50.0
8354
100.0
16925
150.0
25218
200.0
33584
250.0
42002
The sample was prepared by placing a single analgesic tablet in a small beaker and
adding 10 mL of methanol. After allowing the sample to dissolve, the contents of the
beaker, including the insoluble binder, were quantitatively transferred to a 25-mL
volumetric flask and diluted to volume with methanol. The sample was then filtered,
and a 1.00-mL aliquot was transferred to a 10-mL volumetric flask and diluted to
volume with methanol. When analyzed by HPLC, the signal for the caffeine was
found to be 21469. Report the number of milligrams of caffeine in the analgesic
tablet.

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Ksp= 2.3x10^-13