Aseptic Lab Report 2023 Completed
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Aseptic Technique Lab Report
Name: Heather Morales
Date:________________
Are you submitting a photo of your two nutrient agar slant tubes as well as your two nutrient agar plate
transfers with this lab report in Canvas?
__Yes
Lab Report Results:
1.
Which of the above growth patterns most resembles the growth pattern that occurred in your
E. coli
slant?
A. Filiform
B. arborescent
C. beaded
D. effuse
E. rhizoid
F. echinulate
2.
Which of the above growth patterns most resembles the growth that occurred in the
Staphylococcus epidermidis
nutrient agar slant?
A. filiform
B. arborescent
C. beaded
D. effuse
E. rhizoid
F. Echinulate
3.
Examine your two slant cultures.
Which bacterium,
Staphylococcus epidermidis
or
E. coli
,
appeared to have spread out from the inoculated line more along the slant
surface?
a. Staphylococcus
epidermidis
b.
Escherichia coli
4.
Which bacterium,
Staphylococcus epidermidis
or
E. coli
, appeared to be motile (flagellated)?
a. Staphylococcus
epidermidis
b.
Escherichia coli
5.
What is the source of nutrients in the nutrient agar slants and nutrient agar butts?
a. Agar
b.
Glucose and sucrose
c.
Peptones
6.
The growth of an unwanted organism in your culture medium would be called
a. a variant
b.
A contaminant
c.
an inoculum
d.
a virus
7.
When inoculating tubes, remove their caps and place the caps on the table surface.
a. true
b.
False
8.
When transferring bacteria from a solid medium in a petri dish, take the lid off and place it on
the table while doing the transfer so you can have greater freedom of movement.
a. true
b. False
9.
Give an example of a setting in which aseptic technique is necessary?
In an operating room, surgeons and healthcare professionals use aseptic technique
to prevent the introduction of harmful microorganisms into a patient's body during
surgery. They follow strict protocols to maintain a sterile field by using sterile
instruments, wearing sterile gowns and gloves, and creating a controlled
environment to minimize the risk of infections and complications during surgical
procedures.
10.
How could you tell if your transfer cultures were contaminated?
Contamination of cultures can be detected through various observable signs and
changes in the appearances. For instance, color changes where the contaminated
microorganism produce pigments or metabolites that differ from the target
microorganism.
11.
What is the purpose of agar
in a nutrient medium?
a. it provides nutrients for bacterial growth
b.
it inhibits the growth of molds
c. It solidifies a liquid nutrient medium
d.
it prevents the growth of
contaminants
12. What is the advantage of using a plate culture instead of a slant for bacterial
growth?’______________
The primary advantage of using a plate culture is that it provides a large surface
area and the ability to easily isolate and observe bacterial colonies which facilitates
colony counting and quantitative analysis.
13.
What is the advantage of using an agar slant for the growth of bacteria?_____________
It provides a convenient and space-efficient way to store bacterial cultures over an
extended period while maintaining their viability.
14.
Which one would resist dehydration longer? _____________________
An Agar slant
15.
Did your streak plate provide isolated colonies?
Yes,
Did your technique improve?
Yes
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