Aseptic Lab Report 2023 Completed

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School

Lincoln Technical Institute, New Jersey *

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Course

101

Subject

Chemistry

Date

Jan 9, 2024

Type

docx

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3

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Report
Aseptic Technique Lab Report Name: Heather Morales Date:________________ Are you submitting a photo of your two nutrient agar slant tubes as well as your two nutrient agar plate transfers with this lab report in Canvas? __Yes Lab Report Results: 1. Which of the above growth patterns most resembles the growth pattern that occurred in your E. coli slant? A. Filiform B. arborescent C. beaded D. effuse E. rhizoid F. echinulate 2. Which of the above growth patterns most resembles the growth that occurred in the Staphylococcus epidermidis nutrient agar slant? A. filiform B. arborescent C. beaded D. effuse E. rhizoid F. Echinulate 3. Examine your two slant cultures. Which bacterium, Staphylococcus epidermidis or E. coli , appeared to have spread out from the inoculated line more along the slant surface? a. Staphylococcus epidermidis b. Escherichia coli 4. Which bacterium, Staphylococcus epidermidis or E. coli , appeared to be motile (flagellated)? a. Staphylococcus epidermidis b. Escherichia coli 5. What is the source of nutrients in the nutrient agar slants and nutrient agar butts?
a. Agar b. Glucose and sucrose c. Peptones 6. The growth of an unwanted organism in your culture medium would be called a. a variant b. A contaminant c. an inoculum d. a virus 7. When inoculating tubes, remove their caps and place the caps on the table surface. a. true b. False 8. When transferring bacteria from a solid medium in a petri dish, take the lid off and place it on the table while doing the transfer so you can have greater freedom of movement. a. true b. False 9. Give an example of a setting in which aseptic technique is necessary? In an operating room, surgeons and healthcare professionals use aseptic technique to prevent the introduction of harmful microorganisms into a patient's body during surgery. They follow strict protocols to maintain a sterile field by using sterile instruments, wearing sterile gowns and gloves, and creating a controlled environment to minimize the risk of infections and complications during surgical procedures. 10. How could you tell if your transfer cultures were contaminated? Contamination of cultures can be detected through various observable signs and changes in the appearances. For instance, color changes where the contaminated microorganism produce pigments or metabolites that differ from the target microorganism. 11. What is the purpose of agar in a nutrient medium? a. it provides nutrients for bacterial growth b. it inhibits the growth of molds c. It solidifies a liquid nutrient medium d. it prevents the growth of contaminants 12. What is the advantage of using a plate culture instead of a slant for bacterial growth?’______________ The primary advantage of using a plate culture is that it provides a large surface area and the ability to easily isolate and observe bacterial colonies which facilitates colony counting and quantitative analysis. 13. What is the advantage of using an agar slant for the growth of bacteria?_____________
It provides a convenient and space-efficient way to store bacterial cultures over an extended period while maintaining their viability. 14. Which one would resist dehydration longer? _____________________ An Agar slant 15. Did your streak plate provide isolated colonies? Yes, Did your technique improve? Yes
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