PHYS1146 Lab 6 - Copy
pdf
keyboard_arrow_up
School
Northeastern University *
*We aren’t endorsed by this school
Course
1147
Subject
Chemistry
Date
Jan 9, 2024
Type
Pages
9
Uploaded by ElderSnow12035
Report for Experiment #11
Fluid Flow
12/01/23
Abstract
The goal of this experiment was to validate Poiseuille's law. This was done by examining the relationship
between flow rate and pressure, and the relationship between the flow rate and capillary diameter. In
investigation one, a capillary with a diameter of 1.25 mm was used and pressure was adjusted by
changing the height of the fluid reservoir. By using the IPL Straight Line Fit calculator, the slope and
error in slope was obtained and was 0.0289±0.00129. The y-intercept on the graph was equal to 0.1704
and the y-intercept and error found by using the ILP Straight Line Fit calculator and was equal to
0.161±0.0224. The relationship between flow rate and pressure difference was found to be linear, which
validated Poiseuille's law. In investigation two, flow rate through different diameter capillaries was
investigated. There was a linear relationship. The slope value was found to be 3.799±0.114 which was
close to the true value of four, this also validated Poiseuille’s law.
Introduction
A fluid flows due to a difference in pressure and obeys Newton’s laws. Poiseuille’s law of fluid
flow states that the flow rate of a fluid is dependent on the length of a tube, the radius, the change in
pressure, and the viscosity of the fluid. The difference in pressure and flow rate are directly proportional
and flow rate can be defined as the volume of fluid passed per unit of time, in this case volume of water
per second. For laminar flow, the relation between resistance and the capillary’s length and diameter is
written as:
(1)
In this equation, resistance is proportional to capillary length. Flow rate is inversely proportional
to resistance, so when resistance is doubled flow rate is halved. The flow rate is also proportional to the
diameter, so when diameter increases so will the flow rate. The flow rate is inversely proportional to the
viscosity of the fluid. These relationships validate Poiseuille’s law because as stated previously the flow
rate of a fluid is dependent on the length of a tube, the radius, the change in pressure, and the viscosity of
the fluid.
In this lab the relationship between flow rate with changes in pressure and the diameter of the
capillary were examined to validate Poiseuille’s law. In investigation one the time taken for water to flow
through a capillary was measured at different heights. In investigation two the time taken for water to
flow through capillaries with different radii at a constant height was measured. The purpose of this was to
examine the effects height and radius played on the difference in pressure and to investigate Poiseuille’s
law of fluid flow.
Investigation 1
The experimental setup consisted of a fluid flow apparatus, a capillary with a 1.25 mm diameter, a
ruler, a plastic beaker, a metal beaker, a 100 mL graduated cylinder, a stop watch, a large funnel, a metal
rod, and water. The fluid flow apparatus was attached to the metal rod and could be attached to the
capillary. The capillary was held by a clamp. The fluid flow apparatus and the position of the capillary
could be adjusted. The water reservoir had a scale and a baseline level of 6 was chosen. The height
difference of h
2
(height of baseline water level) – h
1
(height of capillary) was equal to 11 cm. The capillary
was then attached to the fluid flow apparatus and water was drained out until no air bubbles were left.
The height of baseline water level was maintained throughout the experiment by replenishing the water.
Once air bubbles were no longer present in the capillary, the 100 mL graduated cylinder with the large
funnel was placed under the capillary and water filled the graduated cylinder until the water level reached
50 mL. The amount of time required for the graduated cylinder to reach 50 mL was recorded with an
iPhone timer, this was done twice. The average time was calculated using the average function in excel.
The flow rate was found by using:
(2)
The flow rate was found for both trials and the average flow rate was found by using the excel
averaging function for the two calculated values. Volume was equal to 50±0.05 mL and the flow rate for t
1
and t
2
were found and then using excel the average flow rate was found. The error in flow rate was
calculated by using:
(3)
R
=
128
η
L
π
d
4
Q
=
∆
V
∆
t
δ
Q
avg
=
δ
t
2
+
δ
V
2
Error in time was the standard deviation of both the time values and was calculated in excel using
the standard deviation function. Error in volume was equal to half of the smallest value of measurement
on the graduated cylinder.
The experiment was repeated three more times with a different
Δ
p each time. The heights of the
fluid flow apparatus and the capillary were adjusted to have height differences of 14 cm, 17 cm, and 20
cm. The average flow rate for each
Δ
p value was calculated by using (3).
Table 1:
Collected data and flow rate of four different pressures.
The baseline height was set to 49.8 cm and the height of the capillary was set to 38.8 cm, 35.8
cm, 32.8 cm, and 29.8 cm for each trial respectively. The average flow rates and error in average flow
rates were found to be 0.480±10.232, 0.574±2.317, 0.628±2.262, and 0.743±0.889 mL/s. A graph of
average flow rate vs change in pressure was made.
Trial
1
2
3
4
Δ
p
11
14
17
20
h1 (cm)
38.8
35.8
32.8
29.8
h2 (cm)
49.8
49.8
49.8
49.8
t1 (s)
97.44
85.53
81.15
67.81
t2 (s)
111.91
88.73
78.03
66.77
avg t (s)
104.675
87.13
79.59
67.29
V (mL)
50
50
50
50
Q1
0.513
0.585
0.616
0.737
Q2
0.447
0.564
0.641
0.749
Qavg
0.480
0.574
0.628
0.743
࠵?
Qavg
10.232
2.317
2.262
0.889
࠵?
h1 cm
0.05
0.05
0.05
0.05
࠵?
h2 cm
0.05
0.05
0.05
0.05
࠵?
Δ
p
0.071
0.071
0.071
0.071
࠵?
V (mL)
0.05
0.5
0.5
0.5
࠵?
t
10.232
2.263
2.206
0.735
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
Fig 1.
- Average flow rate at different
Δ
p
The slope on the graph was found to be 0.0281. By using the IPL Straight Line Fit calculator, the
slope and error in slope was obtained and was 0.0289±0.00129. The y-intercept on the graph was equal to
0.1704 and the y-intercept and error found by using the ILP Straight Line Fit calculator and was equal to
0.161±0.0224. It was expected that the y-intercept would be at zero because with no pressure difference
there should be no flow. The y-intercept calculated, and its uncertainty did not overlap with zero which
would indicate there was some inaccuracy in the measurements of flow. The data does show a strong
linear relationship between pressure and flow, confirming that Poiseuille’s law is valid. There may have
been random errors in the recording of the time and the estimation of the volume at which the time was
taken.
Fig 2.
- Slope and error in slope values obtained by the IPL Straight Line Fit calculator.
Investigation 2
The experimental setup consisted of a fluid flow apparatus, capillaries with a 1.5, 1.25, 1.0, and
0.5 mm diameters, a ruler, a plastic beaker, a metal beaker, a 100 mL graduated cylinder, a 50 mL
graduated cylinder a stop watch, a large funnel, a metal rod, and water. The fluid flow apparatus was
attached to the metal rod and could be attached to the capillary. The capillary was held by a clamp. The
fluid flow apparatus and the position of the capillary could be adjusted. The water reservoir had a scale
and a baseline level of 6 was chosen. The height difference of h
2
(height of baseline water level) – h
1
(height of capillary) was equal to 20 cm besides the last trial where height difference was equal to 50 cm.
Data for the 1.25 mm diameter trial was taken from the values calculated in investigation one. After
copying and pasting the data from investigation one, the capillary was changed to the 1.5 mm diameter
and the capillary was then attached to the fluid flow apparatus and water was drained out until no air
bubbles were left.
The height of baseline water level was maintained throughout the experiment by replenishing the
water. Once air bubbles were no longer present the 100 mL graduated cylinder with the large funnel was
placed under the capillary and water filled the graduated cylinder until the water level reached 50 mL.
The amount of time required for the graduated cylinder to reach 50 mL was recorded with an iPhone
timer, this was done three times. The average time was calculated using the average function in excel. The
flow rate was found by using (2) and average flow rate was found by using the averaging function on
excel for the three flow rate values. The volume was equal to 50±0.05 mL and the error in flow rate was
calculated by using (3).
This process was repeated with a capillary of 1.0 mm diameter. After values for the 1.0 mm
diameter were found, the capillary was changed to the 0.5 mm diameter. However, in this trial the volume
was changed to 10 mL rather than 50 mL and the height difference was set to 50 cm. This was done in
order to obtain data in a reasonable time. After these changes the process for collecting data was repeated.
Table 2:
Collected data and flow rate of four different diameters.
Trial
1
2
3
4
d(mm)
0.5
1
1.25
1.5
࠵?
d (cm)
0.02
0.02
0.02
0.02
Δ
p (cm)
50
20
20
20
h1 (cm)
19
29.8
29.8
29.8
h2 (cm)
69
49.8
49.8
49.8
t1 (s)
167.16
75.06
67.81
33.12
t2 (s)
172.7
74.31
66.77
33.43
t3 (s)
171.57
75.41
x
33.43
avg t (s)
170.477
74.927
67.29
33.327
V (mL)
10
50
50
50
Q1
0.060
0.666
0.737
1.510
Q2
0.058
0.673
0.749
1.496
Q3
0.058
0.663
x
1.496
Due to the pressure difference in the 0.5 mm trial being different than the other trials, the flow
rate was corrected by multiplying the calculated value by
. After this correction the
Δ
p was the same in
all trials and Poiseuille’s equation could be rewritten as:
(4)
In this equation C is equal to
. By taking the logarithm on both sides the equation could
be rewritten as:
(5)
The natural log for average flow rate and diameter was calculated by using the excel function.
Error in natural log of average flow rate and natural log of the diameter was calculated using:
(6)
This equation was provided by the lab procedure and A represented a measured quantity. The
diameters for each trial were 0.5± 0.02 mm, 1.0± 0.02 mm, 1.25± 0.02 mm, and 1.5± 0.02 mm. The
average time for the trials were found to be 170.477 s, 74.927 s, 67.290 s, and 33.327 s. The average flow
rates were found to be 0.023±2.928 mL/s, 0.667±0.752 mL/s, 0.743±0.889 mL/s, and 1.500±0.531 mL/s.
The natural log of the flow rates were found to be –3.752±124.76, -0.404±1.127, -0.297±1.197, and
0.406±0.354. The natural log of the diameters were found to be –0.693±0.04, 0.000±0.02, 0.223±0.016,
and 0.405±0.013. A graph of ln Q vs ln d was then made in excel.
Qavg
0.023
0.667
0.743
1.500
࠵?
Qavg
2.928
0.752
0.889
0.531
lnQavg
-3.752
-0.404
-0.297
0.406
࠵?
lnQavg
124.76
1.127
1.197
0.354
lnd
-0.693
0.000
0.223
0.405
࠵?
lnd
0.04
0.02
0.016
0.013
࠵?
h1
0.05
0.05
0.05
0.05
࠵?
h2
0.05
0.05
0.05
0.05
࠵?
Δ
p
0.071
0.071
0.071
0.071
࠵?
V (mL)
0.05
0.5
0.5
0.5
࠵?
t
2.927
0.562
0.735
0.179
2
5
Q
=
Cd
n
π
∆
p
128
η
L
ln
Q
= ln
(
Cd
n
)
=
n
ln
d
+ ln
C
δ
ln
A
=
δ
A
A
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
Fig. 3
– Natural log of flow rates at different natural log diameters.
The slope of this graph was representative of n and was found to be 3.797. The slope and error in
slope was found using the IPL Straight Line Fit calculator was found to be 3.799±0.114. The expected
result for perfect laminar flow described by Poiseuille’s Law is a value of n equals four. The result
obtained was close to the expected result indicating most of the flow was in perfect laminar flow and it
was unlikely there was much turbulent flow. Possible random errors would include inaccuracy of the
measurement of the height, the time, and the volume of fluid at which the time was taken.
Fig 4.
- Slope and error in slope values obtained by the IPL Straight Line Fit calculator.
Conclusion
These experiments were conducted to explore the validity of Poiseuille’s law, by measuring the
effect of changes in pressure and capillary diameter on the flow rate of a fluid. In investigation one a fluid
flow apparatus was set to a height of 49.8 cm and a capillary with a diameter of 1.25 mm was set to four
different heights of 38.8, 35.8, 32.8, and 29.8 cm. The rate at which fluid flowed out of the capillary was
measured for each of these height differences. A graph of average flow rate vs
Δ
p was made to validate
Poiseuille’s law, the slope and error in slope was obtained and was 0.0289±0.00129. The y-intercept on
the graph was equal to 0.1704 and the y-intercept and error found by using the ILP Straight Line Fit
calculator and was equal to 0.161±0.0224. The y-intercept and uncertainty did not overlap with zero,
which was expected. This could have been due to inaccuracy in measurements of flow. The graphical data
did show a linear relationship between flow and pressure, which validated Poiseuille’s law.
In investigation two the fluid flow apparatus was set to a constant height of 20 cm, except for the
final trial where the height was 50 cm, and each trial was done with a capillary of different diameters. The
diameters used were 1.5, 1.25, 1.0, and 0.5 mm. A graph of the average flow rates vs diameters was made,
and the slope was found using the IPL Straight Line Fit calculator was found to be 3.799±0.114. The
expected result for perfect laminar was four. The result was close to four and this also validated
Poiseuille’s law. Possible improvements for this lab would be to ensure that the capillary was held straight
so gravity would not affect the flow rate, increase the number of trials, and improving the accuracy of the
volume and time measurements.
Questions
1.
You go to the hardware store to buy a new 50 ft garden hose. You find you can choose between
hoses of
½
inch and
⅝
inch inner diameter. Compare the rate at which water flows through the
two types of hoses (i.e., the ratio of rates).
a.
Q=Av
b.
Q
1/2
=Q
5/8
c.
d.
0.64
2.
How does the flow rate Q through a capillary change if the viscosity of the fluid in question
doubles? How will it change if the fluid’s density doubles instead?
a.
When the viscosity of a fluid doubles the flow rate decreases by half. When a fluid's
density doubles the flow rate will not change.
3.
Calculate the ratio Rtube/Rcap for the setup in Investigation 1, where Rtube is the resistance of
the tube connecting the reservoir and capillary and Rcap is the resistance of the capillary. What
conclusion can you draw from this?
a.
A
1
2
v
1
2
=
A
5
8
v
5
8
v
5
8
v
1
2
=
A
1
2
A
5
8
=
π
(
0.5
2
)
2
π
(
0.625
2
)
2
=
R
tube
=
67
cm
(0.7
cm
)
4
= 279.05
b.
c.
d.
The capillary has a higher resistance than the tube therefore …
4.
Now compute the ratio
Δ
ptube/
Δ
pcap, where
Δ
ptube is the pressure drop over the tube and
Δ
pcapis the pressure drop over the capillary.
a.
= 6566
b.
= 686
c.
9.57:1
5.
Why must the capillary in this experiment be positioned horizontally?
a.
The capillary was positioned vertically so no external forces would act on the capillary
and affect the flow rate of the water.
Acknowledgments
References
[1]
Hyde, Batishchev, and Altunkaynak, Introductory Physics Laboratory, pp 80-86, Macmillan Higher
Education, 2022.
[2] IPL Lab Report Guide,
https://web.northeastern.edu/ipl/wp-content/uploads/2017/09/IPL-Lab-Report-
Guide.pdf
[3] Giancoli, Physics Principles with Applications, Pearson, 2021.
R
cap
=
23.9
cm
(
0.125
cm
)
4
= 97894.4
R
tube
R
cap
=
279.05
97894.4
= 0.00285
∆
p
tube
= (1000)(9.8)
(
0.67
)
∆
p
cap
= (1000)(9.8)(0.07)
6566
686
=
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
Related Documents
Related Questions
Calculate the weight % of each component in an unknown sample of volatiles using Area Normalization. Given the following information use ethanol as the internal standard.
Standard
Unknown sample
Component
Weight used (mg)
Raw Peak Area
Component
Raw Peak Area
Ethanol
498.3
5104
Ethanol
3495
Hexane
756.4
9285
Hexane
3267
Benzene
412.7
4374
Benzene
2894
Ethyl acetate
628.9
7239
Ethyl acetate
5402
Calculate the absolute error between the normalized area percentages and the calculated weight percentages of the unknown components.
arrow_forward
Calculate the weight % of each component in an unknown sample of volatiles using Area Normalization. Given the following information use ethanol as the internal standard.
Standard
Unknown sample
Component
Weight used (mg)
Raw Peak Area
Component
Raw Peak Area
Ethanol
498.3
5104
Ethanol
3495
Hexane
756.4
9285
Hexane
3267
Benzene
412.7
4374
Benzene
2894
Ethyl acetate
628.9
7239
Ethyl acetate
5402
Calculate the absolute error between the normalized area percentages and the calculated weight percentages of the unknown components.
arrow_forward
What effect will the following have on plate height (H). Make sure to explain your full and complete reasoning.
Increasing stationary phase thickness.
Reducing the rate of sample injection.
Increasing mobile phase flow rate.
Decreasing temperature.
Reducing stationary phase particle size.
arrow_forward
calculate the kovats retention indices for solutes 1 and 2 from the data shown and assume tm=0.50 min (methane)
arrow_forward
Please don't provide handwritten solution ......
arrow_forward
What is gradient elution?
a. Variation of the column temperature during elution
b. Variation of the stationary phase composition during elution
c. Variation of the mobile phase composition during elution
d. Variation of the mobile phase flow rate during elution
arrow_forward
None
arrow_forward
60% aqueous acetonitrile is isoelutropic with what percent of aqueous methanol?
arrow_forward
Is longitudinal diffusion of the solute a more serious problem in gas or in liquid chromatography?
gas chromatography
O liquid chromatography
Why is longitudinal diffusion a more serious problem in this type of chromatography?
Gas chromatography is carried out at higher temperatures than liquid chromatography.
The solute must be volatile for application of gas chromatography.
The linear velocity in gas chromatography is greater than the linaer velocity in liquid chromatography.
Diffusion coefficients of gases are much greater than those of liquids.
arrow_forward
I have a question with the answer but I don't really understand it. Can you please explain the logic in this question & answer?
For example, the hormone estradiolhas a partition coefficient of 3.2 between 1-octanol and water. How many extractions would need to be completed to recover > 99% from 250 mL of saturated solution using 100 mL of 1-octanol per extraction? The variables given are listed below along with the solution. In this scenario, it would require 5 extractions to recover > 99% of estradiol! Typically extractions are carried out only 2 or 3 times because the partition coefficient is usually larger than 3.2 and there are equal amounts of organic solvent and the aqueous layer.
arrow_forward
Optimize the gradient program for the following separation (do not change any column parameters). You must show all calculations and formulas used.
Separation of A, B, & C with initial mobile phase conditions of 10/90 v/v% MeOH/water, was increased to be 20/80 methanol/water at 4.50 min; then to 40/60 v/v% methanol/water at 6.90 min; and 60/40 v/v% methanol/water at 11.50 min and held constant for 2.50 min.
Compounds A, B, C had capacity factors of 3.50, 5.70, and 11.00 and peak widths at base of 0.80 min, 0.90 min, and 1.20 min, respectively. Void time was 1.00 min. Optimize the separation using an ACN/water mobile phase gradient.
arrow_forward
A researcher was attempting to quantify the amount of dichlorodiphenyltrichloroethane (DDT) in spinach using gas chromatography mass spectrometry (GCMS). First attempts using external calibration proved difficult, as reproducibility and external standard agreement were poor. To overcome these problems, the researcher used the internal standard calibration method with chloroform as the internal standard.
To begin, the researcher analyzed a sample containing 5.67 mg/L DDT standard and 1.84 mg/L chloroform as the internal standard, which produced peak areas of 4035 and 10177 for the DDT and chloroform, respectively.
After establishing the response factor of the instrument, the researcher collected 11.53 g of spinach, homogenized the sample, and extracted the DDT using an established method, producing a 3.70 mL solution containing an unknown amount of extracted DDT. The researcher then prepared a sample for analysis that contained 1.00 mL of the unknown DDT solution and 2.00 mL of 10.25…
arrow_forward
If the radius of column 0.25 cm, the linear.3
velocity of mobile phase 10.0 cm/min, and the
fraction of the column accessible to the mobile
phase 0.50, then the flow rate of the mobile
phase
??
arrow_forward
hree components in a mixture have varying distribution constants between the mobile phase and the stationary phass
One of the components has a very low distribution constant, Would this component likely elute first or last and how
would you design an experiment or modify the components of the separation to make it perform in the opposite
manner?
arrow_forward
Paraxylene, styrene, toluene, and benzene are to be separated with the array of distillation columns
shown in the following figure. Calculate the molar flow rates of D1, D2, B1, and B2.
6% Xylane
5% Styrene
51% Toluene
38% Benzene
19% Xylene
21% Styrene
36% Tolvene
24% Berazene
22% Xylene
B, 20% Styrene
46% Tohvene
12% Benzene
D, 18% Xylene
9% Styrene
42% Tohene
31% Benzene
F= 70 kg-molimin
B
28% Xylene
63% Styrene
8% Tohuene
1% Benzene
a- Using the polymath software.
b- Using the Excel solver.
c- Using Gauss Elimination method.
d- Find the inverse by Gauss Jordan then find the solution of the system.
e- Using LU factorization.
arrow_forward
In normal-phase chromatography, the stationary phase is
Therefore, the eluent strength of the solvent increases as the solvent becomes
less) polar, because the solute is more soluble in the mobile phase as the polarity of the mobile phase
-Energy
(polar / non-polar).
(increase / decrease).
Circle the peak in the spectrum below that corresponds to the circled transition shown in the energy level
diagram.
E₁
⇒ 2
(more /
1 (s
arrow_forward
please quickly ,within 1hour thanks ! If it takes longer than an hour, i don't need the answer
Draw a flowchart for gas chromatography instrument, then list the operationalparameters with an example of analysis application.
arrow_forward
SEE MORE QUESTIONS
Recommended textbooks for you

Chemistry
Chemistry
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning

Chemistry
Chemistry
ISBN:9781259911156
Author:Raymond Chang Dr., Jason Overby Professor
Publisher:McGraw-Hill Education

Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning

Organic Chemistry
Chemistry
ISBN:9780078021558
Author:Janice Gorzynski Smith Dr.
Publisher:McGraw-Hill Education

Chemistry: Principles and Reactions
Chemistry
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:Cengage Learning

Elementary Principles of Chemical Processes, Bind...
Chemistry
ISBN:9781118431221
Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:WILEY
Related Questions
- Calculate the weight % of each component in an unknown sample of volatiles using Area Normalization. Given the following information use ethanol as the internal standard. Standard Unknown sample Component Weight used (mg) Raw Peak Area Component Raw Peak Area Ethanol 498.3 5104 Ethanol 3495 Hexane 756.4 9285 Hexane 3267 Benzene 412.7 4374 Benzene 2894 Ethyl acetate 628.9 7239 Ethyl acetate 5402 Calculate the absolute error between the normalized area percentages and the calculated weight percentages of the unknown components.arrow_forwardCalculate the weight % of each component in an unknown sample of volatiles using Area Normalization. Given the following information use ethanol as the internal standard. Standard Unknown sample Component Weight used (mg) Raw Peak Area Component Raw Peak Area Ethanol 498.3 5104 Ethanol 3495 Hexane 756.4 9285 Hexane 3267 Benzene 412.7 4374 Benzene 2894 Ethyl acetate 628.9 7239 Ethyl acetate 5402 Calculate the absolute error between the normalized area percentages and the calculated weight percentages of the unknown components.arrow_forwardWhat effect will the following have on plate height (H). Make sure to explain your full and complete reasoning. Increasing stationary phase thickness. Reducing the rate of sample injection. Increasing mobile phase flow rate. Decreasing temperature. Reducing stationary phase particle size.arrow_forward
- calculate the kovats retention indices for solutes 1 and 2 from the data shown and assume tm=0.50 min (methane)arrow_forwardPlease don't provide handwritten solution ......arrow_forwardWhat is gradient elution? a. Variation of the column temperature during elution b. Variation of the stationary phase composition during elution c. Variation of the mobile phase composition during elution d. Variation of the mobile phase flow rate during elutionarrow_forward
- Nonearrow_forward60% aqueous acetonitrile is isoelutropic with what percent of aqueous methanol?arrow_forwardIs longitudinal diffusion of the solute a more serious problem in gas or in liquid chromatography? gas chromatography O liquid chromatography Why is longitudinal diffusion a more serious problem in this type of chromatography? Gas chromatography is carried out at higher temperatures than liquid chromatography. The solute must be volatile for application of gas chromatography. The linear velocity in gas chromatography is greater than the linaer velocity in liquid chromatography. Diffusion coefficients of gases are much greater than those of liquids.arrow_forward
- I have a question with the answer but I don't really understand it. Can you please explain the logic in this question & answer? For example, the hormone estradiolhas a partition coefficient of 3.2 between 1-octanol and water. How many extractions would need to be completed to recover > 99% from 250 mL of saturated solution using 100 mL of 1-octanol per extraction? The variables given are listed below along with the solution. In this scenario, it would require 5 extractions to recover > 99% of estradiol! Typically extractions are carried out only 2 or 3 times because the partition coefficient is usually larger than 3.2 and there are equal amounts of organic solvent and the aqueous layer.arrow_forwardOptimize the gradient program for the following separation (do not change any column parameters). You must show all calculations and formulas used. Separation of A, B, & C with initial mobile phase conditions of 10/90 v/v% MeOH/water, was increased to be 20/80 methanol/water at 4.50 min; then to 40/60 v/v% methanol/water at 6.90 min; and 60/40 v/v% methanol/water at 11.50 min and held constant for 2.50 min. Compounds A, B, C had capacity factors of 3.50, 5.70, and 11.00 and peak widths at base of 0.80 min, 0.90 min, and 1.20 min, respectively. Void time was 1.00 min. Optimize the separation using an ACN/water mobile phase gradient.arrow_forwardA researcher was attempting to quantify the amount of dichlorodiphenyltrichloroethane (DDT) in spinach using gas chromatography mass spectrometry (GCMS). First attempts using external calibration proved difficult, as reproducibility and external standard agreement were poor. To overcome these problems, the researcher used the internal standard calibration method with chloroform as the internal standard. To begin, the researcher analyzed a sample containing 5.67 mg/L DDT standard and 1.84 mg/L chloroform as the internal standard, which produced peak areas of 4035 and 10177 for the DDT and chloroform, respectively. After establishing the response factor of the instrument, the researcher collected 11.53 g of spinach, homogenized the sample, and extracted the DDT using an established method, producing a 3.70 mL solution containing an unknown amount of extracted DDT. The researcher then prepared a sample for analysis that contained 1.00 mL of the unknown DDT solution and 2.00 mL of 10.25…arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- ChemistryChemistryISBN:9781305957404Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCostePublisher:Cengage LearningChemistryChemistryISBN:9781259911156Author:Raymond Chang Dr., Jason Overby ProfessorPublisher:McGraw-Hill EducationPrinciples of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
- Organic ChemistryChemistryISBN:9780078021558Author:Janice Gorzynski Smith Dr.Publisher:McGraw-Hill EducationChemistry: Principles and ReactionsChemistryISBN:9781305079373Author:William L. Masterton, Cecile N. HurleyPublisher:Cengage LearningElementary Principles of Chemical Processes, Bind...ChemistryISBN:9781118431221Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. BullardPublisher:WILEY

Chemistry
Chemistry
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Cengage Learning

Chemistry
Chemistry
ISBN:9781259911156
Author:Raymond Chang Dr., Jason Overby Professor
Publisher:McGraw-Hill Education

Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning

Organic Chemistry
Chemistry
ISBN:9780078021558
Author:Janice Gorzynski Smith Dr.
Publisher:McGraw-Hill Education

Chemistry: Principles and Reactions
Chemistry
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:Cengage Learning

Elementary Principles of Chemical Processes, Bind...
Chemistry
ISBN:9781118431221
Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:WILEY