Optimize the gradient program for the following separation (do not change any column parameters). You must show all calculations and formulas used. Separation of A, B, & C with initial mobile phase conditions of 10/90 v/v% MeOH/water, was increased to be 20/80 methanol/water at 4.50 min; then to 40/60 v/v% methanol/water at 6.90 min; and 60/40 v/v% methanol/water at 11.50 min and held constant for 2.50 min. Compounds A, B, C had capacity factors of 3.50, 5.70, and 11.00 and peak widths at base of 0.80 min, 0.90 min, and 1.20 min, respectively. Void time was 1.00 min. Optimize the separation using an ACN/water mobile phase gradient.
Optimize the gradient program for the following separation (do not change any column parameters). You must show all calculations and formulas used. Separation of A, B, & C with initial mobile phase conditions of 10/90 v/v% MeOH/water, was increased to be 20/80 methanol/water at 4.50 min; then to 40/60 v/v% methanol/water at 6.90 min; and 60/40 v/v% methanol/water at 11.50 min and held constant for 2.50 min. Compounds A, B, C had capacity factors of 3.50, 5.70, and 11.00 and peak widths at base of 0.80 min, 0.90 min, and 1.20 min, respectively. Void time was 1.00 min. Optimize the separation using an ACN/water mobile phase gradient.
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Optimize the gradient program for the following separation (do not change any column parameters). You must show all calculations and formulas used.
Separation of A, B, & C with initial mobile phase conditions of 10/90 v/v% MeOH/water, was increased to be 20/80 methanol/water at 4.50 min; then to 40/60 v/v% methanol/water at 6.90 min; and 60/40 v/v% methanol/water at 11.50 min and held constant for 2.50 min.
Compounds A, B, C had capacity factors of 3.50, 5.70, and 11.00 and peak widths at base of 0.80 min, 0.90 min, and 1.20 min, respectively. Void time was 1.00 min. Optimize the separation using an ACN/water mobile phase gradient.
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