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You are trying to come up with a drug to inhibit the activity of an enzyme  thought to have a role in liver disease. In the laboratory the enzyme was shown to have a Km of 1.0 x 10-6 M and Vmax of 0.1 micromoles/min.mg measured at room temperature. You developed an uncompetitive inhibitor. In the presence of 5.0 x 10-5 M inhibitor, the apparent Vmax  was determined to be 0.02 micromoles/min.mg. What is the Ki of the inhibitor?

The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values immediately recorded at the optimum wavelength for a period of 5 minutes (absorbance readings taken every 30 seconds).   Protein concentration of serum sample (mg/ml) Change in absorbance at optimum wavelength per minute Control serum (C) 8 -0.04 Diseased serum (D) 7.8 -0.6 1c. Using the molar absorption coefficient of NADH as 6220 M-1 cm-1, and by application of the Beer-Lambert law, estimate the enzyme activity in the two samples (C and D). Express activity as moles per second.  1d. Estimate the specific activity of the two samples (moles per second per microgram).

In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.

concentration of unknown yellow dye (uM)

Which one of the following statements is not correct about MSDS? A It provides single reference for all information about hazardous Chemicals. It is based on the recommendation of the United Nation's Committee of Experts on Globally Harmonized B System of Classification and Labelling of Chemical; It does not contain information on physical and chemical properties of the material, potential hazards of the material and how to work safely with these materials. Material Safety Data sheet forms the important elements of effective Chemical Hazards Communication D System.

Use the calibration curve or linear regression equation to determine the concentration of FD&C blue dye No. 1 in Kool-aid. Food Coloring is 0.026 M in FD&C blue dye No. 1 7.5 x 10 -5 M is 29 mL -> 1000 mL

The spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1

If an analytical method relies on a measurable property of an analyte that can be correlated with the amount of the said analyte, what type of analytical method is it?   Oxidative methods   Instrumental methods   Blank Correction methods   Classical methods

Plot a professional quality standard curve (absorbance vs. protein amount in µg) with an appropriate title and caption underneath the plot from the data give above. The caption should include the best-fit data.

Real life applications of standardization of NaOH.

You are interested to know the Q10 value for mean heart rate (# of beats/10 sec) for a species of crab for the temperature interval of 25°C - 35°C. To find this value, you collect data for these six temperatures and calculate mean heart rate (# of beats/10 sec) for each temperature. Here is the data: 10°C 15°C 20°C 25°C 30°C 35°C Mean Heart Rate (# 23.67 23.89 24.33 27 35.89 37.78 beats/10 sec) What is the Q1o value for heart rate (# of beats/10 seconds) for the temperature interval of 25°C - 35°C? Round your answer to two decimal places.

what is the concentration of the unknown sample using serial diluitions: 0.05M 1.7472nm, 0.025M 0.8569nm, 0.0125M 0.4167nm, 0.00625M 0.1958nm the epsilon value is 0.0101 and l is 1 cm

Describe the basic components of a GC/MS and identify the nature of detection within the system. What is being measured and how are these measured entities be segregated.

How long is a macrocentury, if a lecture period (50.0 min) is close to 1.00 microcentury?

3. In patients with early stage lung cancer, stereotactic body radiation therapy (SBRT) has greatly improved survival in patients unable to undergo surgery. In tumors 100 Gy 10 BED have demonstrated significantly improved survival, when compared with doses <100 Gy 10. What is the BED of the following protocol? Would you suggest increasing the dose, or does it appear adequate, assuming the given information? What would you expect as the most likely side effect of therapy for this patient? 3 fractions of 15 Gy delivered every other day.

After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40, a student found that her best fit linear line for FD&C Red 40 was y = 2,681x + -0.002. Her Kool-Aid sample had an absorbance of 0.337. If 0.55 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her 8-fl oz cup?

The catalytic activity of an insect aminopeptidase was investigatedusing an artificial peptide substrate. The Vmax was 4.0 × 10-7 M/s and the KM was 1.4 × 10-4 M. The enzyme concentration used in the assay was 1.0 × 10-7 M.   What is the value of kcat?     2.86 x 10-3 M     0.25 M/s     4.0 s-1      2.86 x 104 s/M     0.25 s-1

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You work in a research organization that is looking for markers of various diseases that can be used as a diagnostic for the disease. It has been reported in the past that high levels of Cu are found in the sweat of people with cystic fibrosis. One of the research projects is focused on looking for high levels of Cu in samples that can be obtained non-invasively such as saliva, sweat, hair, nails, etc. The lab will analyze large samples for Cu. What instrument would you recommend purchasing to support this work, Atomic absorption spectrophotometer or an inductively coupled plasma atomic spectrophotometer? Explain the basis for your decision.

The amount of caffeine in an analgesic tablet was determined by HPLC using a normal calibration curve. Standard solutions of caffeine were prepared and analyzed using a 10-µL fixed-volume injection loop. Results for the standards are summarized in the following table. Concentration of Standards Signal (arbitrary units) (ppm) 50.0 8354 100.0 16925 150.0 25218 200.0 33584 250.0 42002 The sample was prepared by placing a single analgesic tablet in a small beaker and adding 10 mL of methanol. After allowing the sample to dissolve, the contents of the beaker, including the insoluble binder, were quantitatively transferred to a 25-mL volumetric flask and diluted to volume with methanol. The sample was then filtered, and a 1.00-mL aliquot was transferred to a 10-mL volumetric flask and diluted to volume with methanol. When analyzed by HPLC, the signal for the caffeine was found to be 21469. Report the number of milligrams of caffeine in the analgesic tablet.