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You work in a research organization that is looking for markers of various diseases that can be used as a diagnostic for the disease. It has been reported in the past that high levels of Cu are found in the sweat of people with cystic fibrosis. One of the research projects is focused on looking for high levels of Cu in samples that can be obtained non-invasively such as saliva, sweat, hair, nails, etc. The lab will analyze large samples for Cu. What instrument would you recommend purchasing to support this work, Atomic absorption spectrophotometer or an inductively coupled plasma atomic spectrophotometer? Explain the basis for your decision.

The spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1

3. In patients with early stage lung cancer, stereotactic body radiation therapy (SBRT) has greatly improved survival in patients unable to undergo surgery. In tumors 100 Gy 10 BED have demonstrated significantly improved survival, when compared with doses <100 Gy 10. What is the BED of the following protocol? Would you suggest increasing the dose, or does it appear adequate, assuming the given information? What would you expect as the most likely side effect of therapy for this patient? 3 fractions of 15 Gy delivered every other day.

210 200 270 260 250 What is the probability of finding this particular combination in the population? (Hint - individuals may be homozygous or heterozygous for alleles!) TPOX D5S818 PentaD Allele Size (bps) Frequency Allele Size (bps) Frequency Allele Size (bps) Frequency 6 224 0.0894 7 165 0.0029 5 389 0.0439 7 228 0.0176 8 169 0.0468 6 394 0.0102 8 232 0.3680 9 173 0.0322 7 399 0.0439 9 236 0.1950 10 177 0.0731 8 404 0.1082 10 240 0.0865 11 181 0.2339 9 409 0.1681 11 244 0.2155 12 185 0.3684 10 414 0.0994 12 248 0.0264 13 189 0.2237 11 419 0.1798 13 252 0.0015 14 193 0.0161 12 424 0.1082 Ladder Crime Sce Suspect 1 Suspect 2 Suspect 3 200 195 190 Ladder Crime Sce Suspect 1 Suspect 2 Suspect 3 240 185 420 == 230 180 410 220 175 400 170 165 - 390 450 440 430 160 370 290 TPOX D5S818 PentaD 380 Ladder Crime Sce Suspect 1 Suspect 2 Suspect 3

9. Which kind of relationship does the curve represent??[K/U=1][C=1] A) direct B) linear C) line of best fit D) Indirect

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In order to measure riboflavin in a breakfast cereal, a 5.00-gram sample of the cereal was extracted with acetic acid and filtered. The extract was transferred to a 50.0 mL volumetric flask and diluted to the mark. The sample was transferred to a cuvette and the fluorescence was measured. The instrument read 59 units (Fluorescence units –an arbitrary scale). 24.00 mL of the cereal extract was sample was mixed with 1.0 mL of a riboflavin standard that was 0.500 ug/mL. The fluorescence of this mixture was measured and read 94 fluorescence units. Calculate the concentration of riboflavin per gram of cereal (use µg/gram of cereal).

77 K to FK = C + 273 C = 5/9 (F-32)

You are trying to come up with a drug to inhibit the activity of an enzyme  thought to have a role in liver disease. In the laboratory the enzyme was shown to have a Km of 1.0 x 10-6 M and Vmax of 0.1 micromoles/min.mg measured at room temperature. You developed an uncompetitive inhibitor. In the presence of 5.0 x 10-5 M inhibitor, the apparent Vmax  was determined to be 0.02 micromoles/min.mg. What is the Ki of the inhibitor?

Case #5 Case #6 Jeepers! Someone snuck into the Movie Madness studio and Someone stole the net from the High Wire act at the Crazy Circus! Without it, the show can't go on! stole the reels for the new film! The CSI team found a liquid at At this crime scene, there were traces of a liquid, 9 drops (at ,059 drop) were found to contain 279C, 119790, and ,06039H, What was the empirical formula of the compound? Who committed the heinous act? this crime scene, When the ,77009 sample was analyzed, it was found to contain ,04679H and ,724390, What was the empirical formula? Who should the police question? a Case #7 Case #8 Old Man Higgins had his favorite cane taken from him when he was out for a walk through Sinister Park, The CSI team found traces of a uhite powder at this crime scene, To be 61,67%CL, A flame test yielded a yellow flame, indicating the remaining portion was sodium, What was the empirical formula of the compound? What kind of person would steal an antique cane? The original…

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You are interested to know the Q10 value for mean heart rate (# of beats/10 sec) for a species of crab for the temperature interval of 25°C - 35°C. To find this value, you collect data for these six temperatures and calculate mean heart rate (# of beats/10 sec) for each temperature. Here is the data: 10°C 15°C 20°C 25°C 30°C 35°C Mean Heart Rate (# 23.67 23.89 24.33 27 35.89 37.78 beats/10 sec) What is the Q1o value for heart rate (# of beats/10 seconds) for the temperature interval of 25°C - 35°C? Round your answer to two decimal places.

8. Chemical Formula: C9H17NO5 8 1H, broad 7 6 Final Answer 1H, broad 5 سلاد etu 4 PPM 3H s 3 2 9H s 1 there are 3 peaks between 4.00 and 4.30 ppm: a 1H t at 4.3 ppm two 1H t that both happen to be at 4.0 ppm, but are not the same 0

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