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Table #2 Worktable for BCA Assay Standard Water (μl) Tube # 1 2 3 4 5 6 7 8 9 0 125 325 175 325 325 325 400 400 BSA source (μl) 300 stock 375 stock 325 stock 175 of tube #2 325 of tube #3 325 of tube #5 325 of tube #6 100 of tube #7 0 Protein (ug) Add the volumes listed in Table 2 to each tube. Add the water first and then the appropriate standard. Pipette each of the protein samples directly into the water, do not dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein (ug)).

urses/55527/quizzes/401369/take → # 3 Complete the data table using the calculation process you used to complete the previous question. Be sure to keep a copy of the completed table to include in the lab report for this experiment. $ Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 4 1.00 M Acetic Acid volume HC₂H3O2 25.0 mL 25.0 mL 25.0 mL 25.0 mL 25.0 mL Q Search f5 % moles 5 f6 Mole Ratio 6 HC₂H3O2 : NaHCO3 3:1 2:1 *All values should contain three (3) significant digits. 1:1 1:2 1:3 U hp NaHCO3 Molar Mass: 84.007 g/mol moles fg * 99+ 8 a fg mass needed DO 9 f10 ► 11

analyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU) caffeine 1 1 4.67 302.85 aspartame 5 1 7.53 15.83 benzoic acid 1 1 8.14 89.98 saccharin 1 1 1.91 84.86 mixture(add everything above with 1:1:1:1 ratio)   1 4.47 69.58 How to get the concentration of the mixture in this case?

yout References Mailings Review View rt Endnote t Footnote ✓ w Notes es 5 Search Researcher Absorbance 5 4.5 4 3.5 2.5 2 1.5 1 0.5 0 Research 0 Insert Citation 1 1. Construct a standard curve in Excel by plotting concentration (in ppm) on your x-ax (unitless) on your y-axis for your known solutions. Label the axes on the graph and provi title. See Figure 5 in the lab handout for an example. Use a linear trendline to generate a data. Label the graph with the equation and the R2 value. Insert your labeled graph in the Help Manage Sources Style: [APA 2 Bibliography Citations & Bibliography Sciwheel Nitrate Standard Curve 3 4 ty=0.8842x-0.8191 R²=0.9509 Q..**. Concentration Insert Caption 5 6 Insert Table of Figure Update Table Cross-reference Captions ww 7 2. Using the equation from your graph, determine the nitrate concentration (in ppm) in each of samples. Text Predictions: On Accessibility: Investigate O Search 8509 FORGE *

Color BEFORE adding Color AFTER adding Intensity of color AFTER adding FeCl3 Test Tube # FeCl3 FeCl3 #1 (salicylic acid) clear lilac 100% #2 (commercial aspirin A) clear slightly pink 50% #3 (commercial aspirin B) clear lilac 95% #4 (aspirin from your clear pink 20% synthesis above) #5 (control) clear clear 0% Based on this data and the melting point of Aspirin, how pure is the synthesized product?

An analysis where we know what analytes we are looking for is called An analysis where we do not know what analytes are in the sample is called a An example is A) untargeted, confirmation, targeted, Comprehensive drug screen B) targeted, confirmation, untargeted, comprehensive drug screen C) targeted, comprehensive drug screen, untargeted, confirmation analysis. An example is analysis. ▪ True or false: The purpose of the internal standard is to make quantitation of the analyte insensitive to injection errors

researchers working in a chemistry lab were examined anatomical structures of the brain using concentrated formaldehyde solutions. Once their studies concluded there remained 1.5 gallons of the substance and the researcher decided to pour this down the drain. In this case, they directly violet regulations put in place by which office?1. Office of research integrity 2. Institutional animal care and use committee3. Occupational safety and health administration 4. Institutional review board

HDPE and PS are both inert to bases and acids, which plastic would you rather chose to put 1 Liter of 6M HCl for general chemistry lab? Explain why.

. Be sure to understand what the dilutionfactor is for each sample (dilution factor = the number used to determine the actualconcentration of the stock UK after the diluted samples are analyzed) ?

What is the chemical equation for main test and for the confirmation test?

Need complete solutions no need ai solutions okk just solve accurate

Column Chromatography Alumina Chromatography Mixture 9:1 Hexanes:Ether 8:2 Hexanes:Ether 1:1 Hexanes:Acetone Amount Used 3.962 g 0.143 g 9.50 mL 9.50 mL 11.00 mL Additional Observations (Color, etc.) BIU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BIU X₂ X² →

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my unknown # is 5.

Question:  Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC.  -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.

Three students did a chromatography experiment, where Rf = distance of solute / distance of solvent. What could be the possible errors why student 3 had results that are quite far from that of students A and B?

asap please 1. Assume your analytical method requires a volume flow rate of 2.5 lpm. The required volume is 25 liters to 125 liters. Employees work four hours before lunch and four hours after lunch. What will be your sampling plan? Please explain fully.

NaOH is the base (please elaborate) Thank you!

The paper used in chromatography is made of molecules with___parts A polar  B nonpolar

Consider four distinct physical properties: boiling point, melting point, solubility, and density. Which of these physical properties do you think allows thin layer chromatography (TLC) to work? Choose one then, explain your reasoning.

in text form with proper workings and explanation for each and every part and steps with concept and introduction no AI no copy paste remember answer must be in proper format with all working!!!!!!!